Impedancemicrobiologyisamethodthatenablestracingmicrobialgrowthbymeasuringthechangeintheelectricalconductivity.Differentsystems,abletoperformthismeasurement,areavailableincommerceandarecommonlyusedforfoodcontrolanalysisbymeanofmeasuringapointoftheimpedancecurve,defined“timeofdetection.”Withthisworkwewantedtofindanobjectivewaytointerpretthemetabolicsignificanceofimpedancecurvesandproposeitasavalidapproachtoevaluatethepotentialacidifyingperformancesofstarterlacticacidbacteriatobeemployedinmilktransformation.TodothisitwasfirstlyinvestigatedthepossibilitytousetheGompertzequationtodescribethedatacomingfromtheimpedancecurveobtainedbymeanofBacTrac4300®.Lagtime(),maximumspecificM%rate(μmax),andmaximumvalueofM%(Yend)havebeencalculatedand,giventhesimilarityoftheimpedancefittedcurvetothebacterialgrowthcurve,theirmeaninghasbeeninterpreted.PotentialacidifyingperformancesofeightystrainsbelongingtoLactobacillushelveticus,Lactobacillusdelbrueckiisubsp.bulgaricus,Lactococcuslactis,andStreptococcusthermophilusspecieshavebeenevaluatedbyusingthekineticsparameters,obtainedfromExceladd-inDMFitversion2.1.Thenoveltyandimportanceofourfindings,obtainedbymeansofBacTrac4300®,isthattheycanalsobeappliedtodataobtainedfromotherdevices.Moreover,themeaningof,μmax,andYendthatwehaveextrapolatedfromModifiedGompertzequationanddiscussedforlacticacidbacteriainmilk,canbeexploitedalsotootherfoodenvironmentorotherbacteria,assumingthattheycangiveacurveandthatcurveisproperlyfittedwithGompertzequation.

Application of Impedance Microbiology for Evaluating Potential Acidifying Performances of Starter Lactic Acid Bacteria to Employ in Milk Transformation / Bancalari, Elena; Bernini, Valentina; Bottari, Benedetta; Neviani, Erasmo; Gatti, Monica. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 7(2016). [10.3389/fmicb.2016.01628]

Application of Impedance Microbiology for Evaluating Potential Acidifying Performances of Starter Lactic Acid Bacteria to Employ in Milk Transformation

BANCALARI, Elena;BERNINI, Valentina;BOTTARI, Benedetta;NEVIANI, Erasmo;GATTI, Monica
2016

Abstract

Impedancemicrobiologyisamethodthatenablestracingmicrobialgrowthbymeasuringthechangeintheelectricalconductivity.Differentsystems,abletoperformthismeasurement,areavailableincommerceandarecommonlyusedforfoodcontrolanalysisbymeanofmeasuringapointoftheimpedancecurve,defined“timeofdetection.”Withthisworkwewantedtofindanobjectivewaytointerpretthemetabolicsignificanceofimpedancecurvesandproposeitasavalidapproachtoevaluatethepotentialacidifyingperformancesofstarterlacticacidbacteriatobeemployedinmilktransformation.TodothisitwasfirstlyinvestigatedthepossibilitytousetheGompertzequationtodescribethedatacomingfromtheimpedancecurveobtainedbymeanofBacTrac4300®.Lagtime(),maximumspecificM%rate(μmax),andmaximumvalueofM%(Yend)havebeencalculatedand,giventhesimilarityoftheimpedancefittedcurvetothebacterialgrowthcurve,theirmeaninghasbeeninterpreted.PotentialacidifyingperformancesofeightystrainsbelongingtoLactobacillushelveticus,Lactobacillusdelbrueckiisubsp.bulgaricus,Lactococcuslactis,andStreptococcusthermophilusspecieshavebeenevaluatedbyusingthekineticsparameters,obtainedfromExceladd-inDMFitversion2.1.Thenoveltyandimportanceofourfindings,obtainedbymeansofBacTrac4300®,isthattheycanalsobeappliedtodataobtainedfromotherdevices.Moreover,themeaningof,μmax,andYendthatwehaveextrapolatedfromModifiedGompertzequationanddiscussedforlacticacidbacteriainmilk,canbeexploitedalsotootherfoodenvironmentorotherbacteria,assumingthattheycangiveacurveandthatcurveisproperlyfittedwithGompertzequation.
Application of Impedance Microbiology for Evaluating Potential Acidifying Performances of Starter Lactic Acid Bacteria to Employ in Milk Transformation / Bancalari, Elena; Bernini, Valentina; Bottari, Benedetta; Neviani, Erasmo; Gatti, Monica. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 7(2016). [10.3389/fmicb.2016.01628]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2816405
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