The objective of this study was to evaluate the susceptibility of 40 L. monocytogenes strains isolated from seafood and processing environments to 19 antibiotics currently used in veterinary and human therapy. Susceptibility tests were performed by the automated svstem VITEK2. Apart from penicillin, Ampicillin and Trimethoprim- Sulfamethoxazole, for which clinical breakpoint for Listeria susceptibility testing are defined according to the Clinical and Laboratory Standard Institute (CLSI), in the present study the CLSI criteria for staphylococci were applied. This study shows that isolated L. monocytogenes strains are susceptible to the antibiotics commonly used in veterinary and human listeriosis treatment. Very few strains (7,5%) showed a resistance behaviour towards Oxacillin, whereas a variable pattern was showed for Ciprofloxacin and Moxifloxacin. Moreover, an increase in tetracycline resistance, reported by several authors, can not be confirmed in this study, probably due to the diîferent sources of strains isolation. At last, the vITEK2 system represents a rapid and easy-to-use mean for antimicrobial susceptibility test of Listeria monocytogenes. Inconclusion, because of the increase of antimicrobial resistances howed by L. monocytogenes, continuous surveillance of emerging antimicrobial resistance among this pathogen is important to ensure effective treatment of human listeriosis. These data can be used for improve background data on antibiotic resistance of strains isolated from food and food environment, even considering the lack of clinical breakpoint provided by the CLSI.
Sensibilità agli agenti antimicrobici di ceppi di Listeria monocytogenes isolati da alimenti / CONTER M; PALUDI D; MUREDDU A; ZANARDI E.; GHIDINI S; IANIERI A. - In: RIVISTA DELL'ASSOCIAZIONE ITALIANA DEI VETERINARI IGIENISTI. - ISSN 1974-8620. - 1.1(2008), pp. 49-52.