Residui di aminoglicosidi nel latte: analisi di conferma

A fast HPLC-MS/MS method for the determination of 6 different amynoglycosides in milk was developed and validated. The antibiotics were extracted by an acetic acid solution after centrifugation and filtration. The analytes were separated using liquid chromatography, A triple quadrupole mass spectrometer was used in positive-ion mode as a detector via Turbo lonspray interface for electrospray inization (ESI). This methcd was then used over 50 samples randomly chosen within those found positive by the microbial growth inhibition test at the Istituto Zooprofilattico Sperimentale per la Lombardia e l'Emilia Romagna of Brescia between 2003 and 2004.