Celiac disease (CD) is an immune-mediated enteropathy triggered by ingested gluten in genetically susceptible individuals and sustained by both adaptive and innate immune responses. Recent studies in murine macrophages demonstrated that the activation of arginase (ARG) metabolic pathway by gluten peptides contributes to the modulation of intestinal permeability in vitro. Here we characterize the effects of gluten on arginine metabolism and cell polarization in human monocytes, from both healthy and CD subjects; both a simplified enzymatic digestion of gliadin (PTG) and a physiological digestion of whole wheat (WD) have been tested. Results indicate that gluten digests induce the onset of an M2-like phenotype in activated macrophages; more precisely, both isoforms of arginase, ARG1 and ARG2, are induced, likely due to the inhibition of mTOR and the consequent induction of C/EBPβ transcription factor. These effects are independent from the origin of gluten, as well as from the digestive protocol employed; moreover, no statistical difference can be evidenced between healthy and CD patients, excluding a diverse predisposition of CD monocytes to gluten-triggered polarization with respect to healthy immune cells. Overall, the present findings sustain a role for arginase pathway in the immune response elicited by human monocytes towards ingested gluten, that, hence, deserves particular attention when addressing the pathogenesis of CD.

Gluten peptides drive healthy and celiac monocytes toward an M2-like polarization / Barilli, Amelia; Gaiani, Federica; Cirlini, Martina; Ingoglia, Filippo; Visigalli, Rossana; Rotoli, Bianca Maria; Nicola, De’Angelis; Sforza, Stefano; DE ANGELIS, Gian Luigi; Dall'Asta, Valeria; Prandi, Barbara. - In: JOURNAL OF NUTRITIONAL BIOCHEMISTRY. - ISSN 0955-2863. - 54:(2018), pp. 11-17. [10.1016/j.jnutbio.2017.10.017]

Gluten peptides drive healthy and celiac monocytes toward an M2-like polarization

Amelia Barilli;Federica Gaiani;Martina Cirlini;Filippo Ingoglia;Rossana Visigalli;Bianca Maria Rotoli;Stefano Sforza;Gian Luigi De’angelis;Valeria Dall’asta;PRANDI Barbara
2018-01-01

Abstract

Celiac disease (CD) is an immune-mediated enteropathy triggered by ingested gluten in genetically susceptible individuals and sustained by both adaptive and innate immune responses. Recent studies in murine macrophages demonstrated that the activation of arginase (ARG) metabolic pathway by gluten peptides contributes to the modulation of intestinal permeability in vitro. Here we characterize the effects of gluten on arginine metabolism and cell polarization in human monocytes, from both healthy and CD subjects; both a simplified enzymatic digestion of gliadin (PTG) and a physiological digestion of whole wheat (WD) have been tested. Results indicate that gluten digests induce the onset of an M2-like phenotype in activated macrophages; more precisely, both isoforms of arginase, ARG1 and ARG2, are induced, likely due to the inhibition of mTOR and the consequent induction of C/EBPβ transcription factor. These effects are independent from the origin of gluten, as well as from the digestive protocol employed; moreover, no statistical difference can be evidenced between healthy and CD patients, excluding a diverse predisposition of CD monocytes to gluten-triggered polarization with respect to healthy immune cells. Overall, the present findings sustain a role for arginase pathway in the immune response elicited by human monocytes towards ingested gluten, that, hence, deserves particular attention when addressing the pathogenesis of CD.
2018
Gluten peptides drive healthy and celiac monocytes toward an M2-like polarization / Barilli, Amelia; Gaiani, Federica; Cirlini, Martina; Ingoglia, Filippo; Visigalli, Rossana; Rotoli, Bianca Maria; Nicola, De’Angelis; Sforza, Stefano; DE ANGELIS, Gian Luigi; Dall'Asta, Valeria; Prandi, Barbara. - In: JOURNAL OF NUTRITIONAL BIOCHEMISTRY. - ISSN 0955-2863. - 54:(2018), pp. 11-17. [10.1016/j.jnutbio.2017.10.017]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2835393
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 14
social impact