Twenty-one free ranging pigs from three organically managed farms in northern Italy were examined for Toxoplasma gondii infection status by meat juice serology. DNA was extracted from all 21 animals and analysed for T. gondii by multilocus nested PCR-RFLP. Results showed a 95.2% prevalence in serology, while PCR was positive in 57.1% of infected pigs. Genotyping of amplified loci for Type I, Type II and Type I/II patterns, suggests the presence of more than one clonal genotype in circulation in these animals. Results of the present study highlight the high exposure to T. gondii in organic pig farms in Italy, indicating a potential risk for meat consumption.
Detection of Toxoplasma gondii in free-range, organic pigs in Italy using serological and molecular methods / Bacci, Cristina; Vismarra, Alice; Mangia, Carlo; Bonardi, Silvia; Bruini, Ilaria; Genchi, Marco; Kramer, Laura Helen; Brindani, Franco. - In: INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY. - ISSN 0168-1605. - 202:(2015), pp. 54-56. [10.1016/j.ijfoodmicro.2015.03.002.]
Detection of Toxoplasma gondii in free-range, organic pigs in Italy using serological and molecular methods
BACCI, Cristina;VISMARRA, Alice;MANGIA, Carlo;BONARDI, Silvia;BRUINI, Ilaria;GENCHI, Marco;KRAMER, Laura Helen
;BRINDANI, Franco
2015-01-01
Abstract
Twenty-one free ranging pigs from three organically managed farms in northern Italy were examined for Toxoplasma gondii infection status by meat juice serology. DNA was extracted from all 21 animals and analysed for T. gondii by multilocus nested PCR-RFLP. Results showed a 95.2% prevalence in serology, while PCR was positive in 57.1% of infected pigs. Genotyping of amplified loci for Type I, Type II and Type I/II patterns, suggests the presence of more than one clonal genotype in circulation in these animals. Results of the present study highlight the high exposure to T. gondii in organic pig farms in Italy, indicating a potential risk for meat consumption.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
