The analysis to detect antimicrobial residues in milk are usually performed in two steps: first, a microbiological method, based on the inibition of microbial growth, is used as a screening tool. Second the samples found positive are confirmed by a chernical method. A confirmatory method has to be able to detect which molecule is present in the sample and to quantitate it. HPLC-UV is the technique usually adopted as a confirmatory method. The use of this technique has some limitations. We developed a very fast chernical method based on HPLC-MS/MS. This method is able to process a great number of samples per day, giving an error-proof confirmation and with a very high sensitivity. The method has been validated and tested on 27 bovine milk samples found positive by a microbioiogical method at the IZSLER of Brescia.

Residues of b-lactam antibiotics in bovine milk: HPLC-MS/MS confirmatory analysis after microbial assay screening / GHIDINI S.; ZANARDI E.; CHIZZOLINI R.; BENEDETTI M.; VARISCO G.. - (2001), pp. 93-95. ((Intervento presentato al convegno 2nd Euroconference Food Safety Assurance and Veterinary Public Health tenutosi a Vienna (Austria) nel 29 Settembre-1 Ottobre 2001.

Residues of b-lactam antibiotics in bovine milk: HPLC-MS/MS confirmatory analysis after microbial assay screening

GHIDINI, Sergio;ZANARDI, Emanuela;CHIZZOLINI, Roberto;
2001

Abstract

The analysis to detect antimicrobial residues in milk are usually performed in two steps: first, a microbiological method, based on the inibition of microbial growth, is used as a screening tool. Second the samples found positive are confirmed by a chernical method. A confirmatory method has to be able to detect which molecule is present in the sample and to quantitate it. HPLC-UV is the technique usually adopted as a confirmatory method. The use of this technique has some limitations. We developed a very fast chernical method based on HPLC-MS/MS. This method is able to process a great number of samples per day, giving an error-proof confirmation and with a very high sensitivity. The method has been validated and tested on 27 bovine milk samples found positive by a microbioiogical method at the IZSLER of Brescia.
Residues of b-lactam antibiotics in bovine milk: HPLC-MS/MS confirmatory analysis after microbial assay screening / GHIDINI S.; ZANARDI E.; CHIZZOLINI R.; BENEDETTI M.; VARISCO G.. - (2001), pp. 93-95. ((Intervento presentato al convegno 2nd Euroconference Food Safety Assurance and Veterinary Public Health tenutosi a Vienna (Austria) nel 29 Settembre-1 Ottobre 2001.
File in questo prodotto:
File Dimensione Formato  
euroconferenceVienna2001RESIDUES.PDF

non disponibili

Tipologia: Documento in Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 368.8 kB
Formato Adobe PDF
368.8 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/1464014
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact