: In this work, the Longissimus thoracis pig skeletal muscle was used as a model to investigate the impact of two different diets, supplemented with n-3 polyunsaturated fatty acids from extruded linseed (L) and polyphenols from grape skin and oregano extracts (L+P), on the lipidomic profile of meat. A standard diet for growing-finishing pigs (CTRL) was used as a control. Changes in lipids profile were investigated through an untargeted lipidomics and transcriptomics combined investigation. The lipidomics identified 1507 compounds, with 195 compounds fitting with the MS/MS spectra of LipidBlast database. When compared with the CTRL group, the L+P diet significantly increased 15 glycerophospholipids and 8 sphingolipids, while the L diet determined a marked up-accumulation of glycerolipids. According to the correlations outlined between discriminant lipids and genes, the L diet may act preventing adipogenesis and the related inflammation processes, while the L+P diet promoted the expression of genes involved in lipids' biosynthesis and adipogenic extracellular matrix formation and functioning.

A molecular insight into the lipid changes of pig Longissimus thoracis muscle following dietary supplementation with functional ingredients / Rocchetti, Gabriele; Vitali, Marika; Zappaterra, Martina; Righetti, Laura; Sirri, Rubina; Lucini, Luigi; Dall'Asta, Chiara; Davoli, Roberta; Galaverna, Gianni. - In: PLOS ONE. - ISSN 1932-6203. - 17:3(2022), p. e0264953. [10.1371/journal.pone.0264953]

A molecular insight into the lipid changes of pig Longissimus thoracis muscle following dietary supplementation with functional ingredients

Righetti, Laura
;
Dall'Asta, Chiara;Galaverna, Gianni
2022

Abstract

: In this work, the Longissimus thoracis pig skeletal muscle was used as a model to investigate the impact of two different diets, supplemented with n-3 polyunsaturated fatty acids from extruded linseed (L) and polyphenols from grape skin and oregano extracts (L+P), on the lipidomic profile of meat. A standard diet for growing-finishing pigs (CTRL) was used as a control. Changes in lipids profile were investigated through an untargeted lipidomics and transcriptomics combined investigation. The lipidomics identified 1507 compounds, with 195 compounds fitting with the MS/MS spectra of LipidBlast database. When compared with the CTRL group, the L+P diet significantly increased 15 glycerophospholipids and 8 sphingolipids, while the L diet determined a marked up-accumulation of glycerolipids. According to the correlations outlined between discriminant lipids and genes, the L diet may act preventing adipogenesis and the related inflammation processes, while the L+P diet promoted the expression of genes involved in lipids' biosynthesis and adipogenic extracellular matrix formation and functioning.
A molecular insight into the lipid changes of pig Longissimus thoracis muscle following dietary supplementation with functional ingredients / Rocchetti, Gabriele; Vitali, Marika; Zappaterra, Martina; Righetti, Laura; Sirri, Rubina; Lucini, Luigi; Dall'Asta, Chiara; Davoli, Roberta; Galaverna, Gianni. - In: PLOS ONE. - ISSN 1932-6203. - 17:3(2022), p. e0264953. [10.1371/journal.pone.0264953]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2920151
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