Pectic oligosaccharides (POS) have been indicated as novel candidate prebiotics. Traditionally, POS are produced from pectin-rich by-products using a two-step process involving extraction of the pectin, followed by its hydrolysis into POS. A one-step approach, in which the POS is directly produced from the raw material, might provide a more efficient alternative. Thus, the main aim of this paper was to investigate a one-step enzymatic hydrolysis approach to directly produce POS from sugar beet pulp (SBP). The POS yield was investigated as a function of the process parameters, as well as raw material characteristics. A statistically-based response surface methodology, using a central composite design was applied, to investigate the individual as well as the combined influences of the diverse parameters. The model was confirmed by a validation experiment, carried out at 135Â g/l substrate concentration, 0.75 FPU/gÂ SBP enzyme concentration, 0.8Â mm particle size and 3Â h hydrolysis time. Under these conditions, a POS-rich hydrolysate was obtained, containing rhamnose, arabinose, galactose, xylose and galacturonic acid, at 0.9, 15.2, 5.1, 1.4, and 13.2Â g/l, respectively, enzymes were added each at 20 FPU/g dry matter (DM).
Enzymatic pectic oligosaccharides (POS) production from sugar beet pulp using response surface methodology / Babbar, Neha; Dejonghe, Winnie; Sforza, Stefano; Elst, Kathy. - In: JOURNAL OF FOOD SCIENCE AND TECHNOLOGY. - ISSN 0022-1155. - 54:11(2017), pp. 3707-3715. [10.1007/s13197-017-2835-x]
|Appare nelle tipologie:||1.1 Articolo su rivista|