The success of immune checkpoint inhibitors strengthens the notion that tumor growth and regression are immune regulated. To determine whether distinct tissue immune microenvironments differentially impact on clinical outcome in Non Small Cell Lung Cancer (NSCLC), an extended analysis of PD-L1 and Tumor Infiltrating Lymphocytes (TILs) was performed. Experimental Design Samples from resected adenocarcinoma (ADC 42) and squamous cell carcinoma (SCC 58) and from 26 advanced diseases (13 ADC, 13 SCC) treated with nivolumab were analyzed. PD-L1 expression and the incidence of CD3, CD8, CD4, PD-1, CD57, FOXP3, CD25 and Granzyme B TILs was immunohistochemically assessed. Results PD-L1 levels inversely correlated with N involvement although did not show a statistical significant prognostic value in resected patients. The incidence and phenotype of TILs differed in SCC vs ADC in which EGFR and KRAS mutations conditioned a different frequency and tissue localization of lymphocytes. NSCLC resected patients with high CD8 pos lymphocytes lacking PD-1 inhibitory receptor had a longer Overall Survival (OS:HR=2.268, 95%CI 1.056-4.871,p=0.03). PD-1-to-CD8 ratio resulted a prognostic factor both on univariate (HR=1.952, 95%CI 1.34-3.12,p=0.001) and multivariate (HR=1.943, 95%CI 1.38-2.86,p=0.009) analysis. Moreover, low PD-1 incidence among CD8(pos) cells was a distinctive feature of nivolumab treated patients showing clinical benefit with a prolonged Progression-Free Survival (PFS:HR=4.51, 95%CI 1.45-13.94,p=0.004). Conclusions In the presence of intrinsic variability in PD-L1 expression, the reservoir of PD-1 negative effector T-lymphocytes provides an immune-privileged microenvironment with a positive impact on survival of patients with resected disease and response to immunotherapy in advanced NSCLC.

Low PD-1 expression in Cytotoxic CD8+ Tumor infiltrating Lymphocytes Confers an Immune Privileged Tissue Microenvironment in NSCLC with a Prognostic and Predictive Value / Mazzaschi, Giulia; Madeddu, Denise; Falco, Angela; Bocchialini, Giovanni; Goldoni, Matteo; Sogni, Francesco; Armani, Giovanna; Lagrasta, Costanza Anna maria; Lorusso, Bruno; Mangiaracina, Chiara Maria; Vilella, Rocchina; Frati, Caterina; Alfieri, Roberta; Ampollini, Luca; Veneziani, Michele; Silini, Enrico Maria; Ardizzoni, Andrea; Urbanek, Konrad Arkadiusz; Aversa, Franco; Quaini, Federico; Tiseo, Marcello. - In: CLINICAL CANCER RESEARCH. - ISSN 1078-0432. - 24:2(2018), pp. 407-419. [10.1158/1078-0432.CCR-17-2156]

Low PD-1 expression in Cytotoxic CD8+ Tumor infiltrating Lymphocytes Confers an Immune Privileged Tissue Microenvironment in NSCLC with a Prognostic and Predictive Value

Mazzaschi, Giulia;Madeddu, Denise;Falco, Angela;Bocchialini, Giovanni;Goldoni, Matteo;Sogni, Francesco;Lagrasta, Costanza Anna maria;Lorusso, Bruno;Mangiaracina, CHIARA MARIA;Vilella, Rocchina;Frati, Caterina;Alfieri, Roberta;Ampollini, Luca;Veneziani, Michele;Silini, Enrico Maria;Urbanek, KONRAD ARKADIUSZ;Aversa, Franco;Quaini, Federico;Tiseo, Marcello
2018

Abstract

The success of immune checkpoint inhibitors strengthens the notion that tumor growth and regression are immune regulated. To determine whether distinct tissue immune microenvironments differentially impact on clinical outcome in Non Small Cell Lung Cancer (NSCLC), an extended analysis of PD-L1 and Tumor Infiltrating Lymphocytes (TILs) was performed. Experimental Design Samples from resected adenocarcinoma (ADC 42) and squamous cell carcinoma (SCC 58) and from 26 advanced diseases (13 ADC, 13 SCC) treated with nivolumab were analyzed. PD-L1 expression and the incidence of CD3, CD8, CD4, PD-1, CD57, FOXP3, CD25 and Granzyme B TILs was immunohistochemically assessed. Results PD-L1 levels inversely correlated with N involvement although did not show a statistical significant prognostic value in resected patients. The incidence and phenotype of TILs differed in SCC vs ADC in which EGFR and KRAS mutations conditioned a different frequency and tissue localization of lymphocytes. NSCLC resected patients with high CD8 pos lymphocytes lacking PD-1 inhibitory receptor had a longer Overall Survival (OS:HR=2.268, 95%CI 1.056-4.871,p=0.03). PD-1-to-CD8 ratio resulted a prognostic factor both on univariate (HR=1.952, 95%CI 1.34-3.12,p=0.001) and multivariate (HR=1.943, 95%CI 1.38-2.86,p=0.009) analysis. Moreover, low PD-1 incidence among CD8(pos) cells was a distinctive feature of nivolumab treated patients showing clinical benefit with a prolonged Progression-Free Survival (PFS:HR=4.51, 95%CI 1.45-13.94,p=0.004). Conclusions In the presence of intrinsic variability in PD-L1 expression, the reservoir of PD-1 negative effector T-lymphocytes provides an immune-privileged microenvironment with a positive impact on survival of patients with resected disease and response to immunotherapy in advanced NSCLC.
Low PD-1 expression in Cytotoxic CD8+ Tumor infiltrating Lymphocytes Confers an Immune Privileged Tissue Microenvironment in NSCLC with a Prognostic and Predictive Value / Mazzaschi, Giulia; Madeddu, Denise; Falco, Angela; Bocchialini, Giovanni; Goldoni, Matteo; Sogni, Francesco; Armani, Giovanna; Lagrasta, Costanza Anna maria; Lorusso, Bruno; Mangiaracina, Chiara Maria; Vilella, Rocchina; Frati, Caterina; Alfieri, Roberta; Ampollini, Luca; Veneziani, Michele; Silini, Enrico Maria; Ardizzoni, Andrea; Urbanek, Konrad Arkadiusz; Aversa, Franco; Quaini, Federico; Tiseo, Marcello. - In: CLINICAL CANCER RESEARCH. - ISSN 1078-0432. - 24:2(2018), pp. 407-419. [10.1158/1078-0432.CCR-17-2156]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2834730
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