Redraspberries,containingellagitanninsandcyanidin-basedanthocyanins,werefedtovolunteersand metabolites appearinginplasmaandurinewereanalysedbyUHPLC-MS.Anthocyaninswerenotab- sorbed toanyextentwithsubnmol/Lconcentrationsofcyanidin-3-O-glucoside andacyanidin-O-glucuronide appearingtransientlyinplasma.Anthocyaninsexcretedinurinecorrespondedto0.007%of intake. MoresubstantialamountsofphaseIImetabolitesofferulicacidandisoferulicacid,alongwith4′- hydroxyhippuricacid,potentiallyoriginatingfrompH-mediateddegradationofcyanidinintheproximal gastrointestinaltract,appearedinurineandalsoplasmawherepeakconcentrationswereattained1– 1.5hafterraspberryintake.Excretionof18anthocyanin-derivedmetabolitescorrespondedto15.0%of intake, a figure substantiallyhigherthanobtainedinotheranthocyaninfeedingstudies.Ellagitannins pass fromthesmalltothelargeintestinewherethecolonicmicrobiotamediatetheirconversionto urolithins AandBwhichappearedinplasmaandwereexcretedalmostexclusivelyassulfateandglu- curonide metabolites.Theurolithinmetabolitespersistedinthecirculatorysystemandwereexcretedin urine formuchlongerperiodsoftimethantheanthocyaninmetabolitesalthoughtheiroverallurinary recoverywaslowerat7.0%ofintake.Itiseventsoriginatingintheproximalanddistalgastrointestinal tract, andsubsequentphaseIImetabolism,thatplayanimportantroleinthebioavailabilityofboth anthocyaninsandellagitanninsanditistheirmetaboliteswhichappearinthecirculatorysystem,that are keytoelucidatingthemodeofaction(s)underlyingtheprotectiveeffectsofthesecompoundson human health.
New insights into the bioavailability of red raspberry anthocyanins and ellagitannins / Ludwig, Iziar A; MENA PARRENO, Pedro Miguel; Calani, Luca; Borges, Gina; Pereira Caro, Gema; Bresciani, Letizia; DEL RIO, Daniele; Lean, Michael E. J.; Crozier, Alan. - In: FREE RADICAL BIOLOGY & MEDICINE. - ISSN 0891-5849. - 89:(2015), pp. 758-769. [10.1016/j.freeradbiomed.2015.10.400]
New insights into the bioavailability of red raspberry anthocyanins and ellagitannins
MENA PARRENO, Pedro Miguel;CALANI, Luca;BRESCIANI, Letizia;DEL RIO, Daniele;
2015-01-01
Abstract
Redraspberries,containingellagitanninsandcyanidin-basedanthocyanins,werefedtovolunteersand metabolites appearinginplasmaandurinewereanalysedbyUHPLC-MS.Anthocyaninswerenotab- sorbed toanyextentwithsubnmol/Lconcentrationsofcyanidin-3-O-glucoside andacyanidin-O-glucuronide appearingtransientlyinplasma.Anthocyaninsexcretedinurinecorrespondedto0.007%of intake. MoresubstantialamountsofphaseIImetabolitesofferulicacidandisoferulicacid,alongwith4′- hydroxyhippuricacid,potentiallyoriginatingfrompH-mediateddegradationofcyanidinintheproximal gastrointestinaltract,appearedinurineandalsoplasmawherepeakconcentrationswereattained1– 1.5hafterraspberryintake.Excretionof18anthocyanin-derivedmetabolitescorrespondedto15.0%of intake, a figure substantiallyhigherthanobtainedinotheranthocyaninfeedingstudies.Ellagitannins pass fromthesmalltothelargeintestinewherethecolonicmicrobiotamediatetheirconversionto urolithins AandBwhichappearedinplasmaandwereexcretedalmostexclusivelyassulfateandglu- curonide metabolites.Theurolithinmetabolitespersistedinthecirculatorysystemandwereexcretedin urine formuchlongerperiodsoftimethantheanthocyaninmetabolitesalthoughtheiroverallurinary recoverywaslowerat7.0%ofintake.Itiseventsoriginatingintheproximalanddistalgastrointestinal tract, andsubsequentphaseIImetabolism,thatplayanimportantroleinthebioavailabilityofboth anthocyaninsandellagitanninsanditistheirmetaboliteswhichappearinthecirculatorysystem,that are keytoelucidatingthemodeofaction(s)underlyingtheprotectiveeffectsofthesecompoundson human health.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
