The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.

Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn / MENA PARRENO, Pedro Miguel; Calani, Luca; Dall'Asta, Chiara; Galaverna, Gianni; Garcia Viguera, C; Bruni, Renato; Crozier, A; DEL RIO, Daniele. - In: MOLECULES. - ISSN 1420-3049. - 17:12(2012), pp. 14821-14840. [10.3390/molecules171214821]

Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn

MENA PARRENO, Pedro Miguel;CALANI, Luca;DALL'ASTA, Chiara;GALAVERNA, Gianni;BRUNI, Renato;DEL RIO, Daniele
2012-01-01

Abstract

The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.
2012
Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn / MENA PARRENO, Pedro Miguel; Calani, Luca; Dall'Asta, Chiara; Galaverna, Gianni; Garcia Viguera, C; Bruni, Renato; Crozier, A; DEL RIO, Daniele. - In: MOLECULES. - ISSN 1420-3049. - 17:12(2012), pp. 14821-14840. [10.3390/molecules171214821]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2546644
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