PNA probes for the specific detection of DNA from olive oil samples by microarrays technology were developed. The presence of as low as 5% refined hazelnut (Corylus avellana) oil in extra-virgin olive oil (Olea europaea L.) could be detected by using a PNA microarray. A set of two single nucleotide polymorphisms (SNPs) from the Actin gene of Olive was chosen as a model for evaluating the ability of PNA probes for discriminating olive cultivars. Both unmodified and C2-modified PNAs bearing an arginine side-chain were used, the latter showing higher sequence specificity. DNA extracted from leaves of three different cultivars (Ogliarola leccese, Canino and Frantoio) could be easily discriminated using a microarray with unmodified PNA probes, whereas discrimination of DNA from oil samples was more challenging, and could be obtained only by using chiral PNA probes.
Selective recognition of DNA from olive leaves and olive oil by PNA and modified-PNA microarrays / Rossi, Stefano; Calabretta, Alessandro; Tedeschi, Tullia; Sforza, Stefano; S., Arcioni; L., Baldoni; Corradini, Roberto; Marchelli, Rosangela. - In: ARTIFICIAL DNA, PNA & XNA. - ISSN 1949-095X. - 3:2(2012), pp. 63-72.