Detection and enumeration of L. monocytogenes have been carried out in 92 Ready-to-Eat (RTE) seafood products, manufactured in different countries and marketed in Italy. Fifty-one strains were characterised by serotyping, multiplex PCRs for the presence of virulence genes and PFGE. Of the samples tested, 8.6% were contaminated at a level of less than 100 CFU/g in 98% of the samples. Isolates were serotyped as 1/2a, 1/2b, 1/2c and 4e, and presented all the virulence genes considered. PFGE revealed a high similarity among the isolates, suggesting the existence of a widespread population of L. monocytogenes clones in different geographical areas.
Molecular characterization of L. monocytogenes isolated from ready-to-eat seafood in Italy / Mureddu, A.; Conter, Mauro; Meloni, D.; Piras, F.; Ianieri, Adriana; Mazzette, R.. - In: ITALIAN JOURNAL OF FOOD SCIENCE. - ISSN 1120-1770. - 23:(2011), pp. 106-117.
Molecular characterization of L. monocytogenes isolated from ready-to-eat seafood in Italy.
CONTER, Mauro;IANIERI, Adriana;
2011-01-01
Abstract
Detection and enumeration of L. monocytogenes have been carried out in 92 Ready-to-Eat (RTE) seafood products, manufactured in different countries and marketed in Italy. Fifty-one strains were characterised by serotyping, multiplex PCRs for the presence of virulence genes and PFGE. Of the samples tested, 8.6% were contaminated at a level of less than 100 CFU/g in 98% of the samples. Isolates were serotyped as 1/2a, 1/2b, 1/2c and 4e, and presented all the virulence genes considered. PFGE revealed a high similarity among the isolates, suggesting the existence of a widespread population of L. monocytogenes clones in different geographical areas.| File | Dimensione | Formato | |
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