A monoclonal antibody (antik-B) against an oligopeptide of 23 AA corresponding to the region 131–153 of bovine κ-casein (κ-CN) B was generated using the Human Combinatorial Antibody Library (HuCAL) technology. Both AA substitutions distinguishing κ-CN A and B are located in that region (positions 136 and 148). In this study, the reactivity of antik-B to milk samples collected from cows previously genotyped as CSN3*AA, CSN3*AB, and CSN3*BB was tested. According to Western blot results, antik-B recognized κ-CN B and it showed no cross-reactivity toward κ-CN A and other milk proteins. Furthermore, a modified Western blot method, urea-PAGE Western blot, was set up to assess the reactivity of antik-B toward all isoforms of κ-CN B. In conclusion, antik-B was specific to κ-CN B in milk and it seemed to be reactive toward all its isoforms.
characterization of a monoclonal antibody for kappa-casein B of cow's milk / Summer, Andrea; Santus, E; Casanova, L; Joerg, H; Rossoni, A; Nicoletti, C; Donofrio, Gaetano; Mariani, Primo; Malacarne, Massimo. - In: JOURNAL OF DAIRY SCIENCE. - ISSN 0022-0302. - 93:(2010), pp. 796-800. [10.3168/jds.2009-2636]
characterization of a monoclonal antibody for kappa-casein B of cow's milk.
SUMMER, Andrea;DONOFRIO, Gaetano;MARIANI, Primo;MALACARNE, Massimo
2010-01-01
Abstract
A monoclonal antibody (antik-B) against an oligopeptide of 23 AA corresponding to the region 131–153 of bovine κ-casein (κ-CN) B was generated using the Human Combinatorial Antibody Library (HuCAL) technology. Both AA substitutions distinguishing κ-CN A and B are located in that region (positions 136 and 148). In this study, the reactivity of antik-B to milk samples collected from cows previously genotyped as CSN3*AA, CSN3*AB, and CSN3*BB was tested. According to Western blot results, antik-B recognized κ-CN B and it showed no cross-reactivity toward κ-CN A and other milk proteins. Furthermore, a modified Western blot method, urea-PAGE Western blot, was set up to assess the reactivity of antik-B toward all isoforms of κ-CN B. In conclusion, antik-B was specific to κ-CN B in milk and it seemed to be reactive toward all its isoforms.File | Dimensione | Formato | |
---|---|---|---|
Summer et al 2010 J Dairy Sci.pdf
non disponibili
Tipologia:
Documento in Post-print
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
179.83 kB
Formato
Adobe PDF
|
179.83 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.