Invasion of the DNA snatchers: We show that one strand of decamer DKα-PNA efficiently invades into double-stranded DNA (dsDNA) by utilizing the SSB-assisted duplex invasion. An extensive analysis of DKα-PNA–DNA duplex formation performed by calculation of the thermodynamic parameters and circular dichroism experiments ascertained that a right-handed helical preorganization is a very important contribution when one PNA strand invades into dsDNA
SSB-Assisted Duplex Invasion of Preorganized PNA into Double-StrandedDNA / Ishizuka, T.; Tedeschi, Tullia; Corradini, Roberto; Komiyama, M.; Sforza, Stefano; Marchelli, Rosangela. - In: CHEMBIOCHEM. - ISSN 1439-4227. - 10:(2009), pp. 2607-2612. [10.1002/cbic.200900381]
SSB-Assisted Duplex Invasion of Preorganized PNA into Double-StrandedDNA
TEDESCHI, Tullia;CORRADINI, Roberto;SFORZA, Stefano;MARCHELLI, Rosangela
2009-01-01
Abstract
Invasion of the DNA snatchers: We show that one strand of decamer DKα-PNA efficiently invades into double-stranded DNA (dsDNA) by utilizing the SSB-assisted duplex invasion. An extensive analysis of DKα-PNA–DNA duplex formation performed by calculation of the thermodynamic parameters and circular dichroism experiments ascertained that a right-handed helical preorganization is a very important contribution when one PNA strand invades into dsDNA| File | Dimensione | Formato | |
|---|---|---|---|
|
chembiochem.pdf
non disponibili
Tipologia:
Documento in Post-print
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
539.05 kB
Formato
Adobe PDF
|
539.05 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
