Verocytotoxin-producing Escherichia coli (VTEC) strains can cause severe human diseases, such as haemorrhagic colitis and haemolytic-uraemic-syndrome (HUS), together with mild diarrhoeal diseases and asymptomatic infections. VTEC strains were isolated by the use of immunomagnetic-separation (IMS) and genes coding for virulence factors were identified by multiplex PCR. A high number of strains were found to be positive for VTEC virulence genes by multiplex PCR.
Use of Immunomagnetic Separation Technique and Polymerase Chain Reaction for Detection of Verocytotoxin-producing Escherichia coli in Cattle at Slaughter / Bonardi, Silvia; Bacci, Cristina; Pizzin, G.; Orlandini, B.; Donati, E.; Brindani, Franco; Foni, E.; Chiapponi, C.; Aibino, E.. - In: VETERINARY RESEARCH COMMUNICATIONS. - ISSN 0165-7380. - 29, Suppl. 2:(2005), pp. 323-326. [10.1007/s11259-005-0072-7]
Use of Immunomagnetic Separation Technique and Polymerase Chain Reaction for Detection of Verocytotoxin-producing Escherichia coli in Cattle at Slaughter
BONARDI, Silvia;BACCI, Cristina;BRINDANI, Franco;
2005-01-01
Abstract
Verocytotoxin-producing Escherichia coli (VTEC) strains can cause severe human diseases, such as haemorrhagic colitis and haemolytic-uraemic-syndrome (HUS), together with mild diarrhoeal diseases and asymptomatic infections. VTEC strains were isolated by the use of immunomagnetic-separation (IMS) and genes coding for virulence factors were identified by multiplex PCR. A high number of strains were found to be positive for VTEC virulence genes by multiplex PCR.| File | Dimensione | Formato | |
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