Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently detecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations. For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023).1
FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses / Tiezzi, C.; Rossi, M.; Vecchi, A.; Doselli, S.; Penna, A.; Fisicaro, P.; Montali, I.; Ceccatelli Berti, C.; Reverberi, V.; Montali, A.; Missale, G.; Ferrari, C.; Boni, C.. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 4:4(2023), p. 102584.102584. [10.1016/j.xpro.2023.102584]
FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses
Tiezzi C.;Rossi M.;Ceccatelli Berti C.;Montali A.;Missale G.;Ferrari C.;Boni C.
2023-01-01
Abstract
Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently detecting ex vivo antiviral cytokine production by SARS-CoV-2-specific T cells following peptide stimulation. We then detail intracellular cytokine staining by flow cytometry to further validate the FluoroSpot assay results and define the specific T cell subpopulations. For complete details on the use and execution of this protocol, please refer to Tiezzi et al. (2023).1| File | Dimensione | Formato | |
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