Ovarian 17α-hydroxyprogesterone responses to GnRH analog testing in oligomenorrheic insulin-dependent diabetic

Objective: To investigate the pituitary-ovarian function in adolescent girls with insulin-dependent diabetes mellitus (IDDM). Design: Clinical case- control study. Methods: The GnRH analog leuprolide acetate was administered subcutaneously to 16 adolescents with IDDM (seven eumenorrheic and nine oligomenorrheic) and 13 controls between 0800 and 0900 h. Blood samples were collected at baseline and 0.5, 3, 6 and 24 h after leuprolide to measure levels of gonadotropins, 17 α-hydroxyprogesterone (17-OHP), androgens and estradiol. Results: Mean baseline serum LH levels were significantly higher in eumenorrheic compared with oligomenorrheic IDDm patients, while peak LH responses to GnRH analog testing were similar in all subjects. Oligomenorrheic IDDM girls showed, as a group, a distinct 12-OHP response to GnRH analog stimulation, which in five out of nine girls was in the range of functional ovarian hyperandrogenism (≤ 8.6 nmol/l). Androgen and estradiol levels were not significantly altered in any group. No correlation was found between steroid levels and HbA(1c) levels, although the latter were significantly higher in oligomenorrheic than in eumenorrheic patients. Conclusion: About 50% of the oligomenorrheic IDDm adolescents had an increased ovarian 17-OHP response to GnRH analog stimulation in the range of functional ovarian hyperandrogenism. Factors other than metabolic control, such as stress, may play an etiologic role in IDDM ovarian dysfunction.