Phosphoserine phosphatase (PSP) catalyzes the final step of de novo L-serine biosynthesis— the hydrolysis of phosphoserine to serine and inorganic phosphate—in humans, bacteria, and plants. In published works, the reaction is typically monitored through the discontinuous malachite green phosphate assay or, more rarely, through a continuous assay that couples phosphate release to the phosphorolysis of a chromogenic nucleoside by the enzyme purine nucleoside phosphorylase (PNP). These assays suffer from numerous drawbacks, and both rely on the detection of phosphate. We describe a new continuous assay that monitors the release of serine by exploiting bacterial serine acetyltransferase (SAT) as a reporter enzyme. SAT acetylates serine, consuming acetyl-CoA and releasing CoA-SH. CoA-SH spontaneously reacts with Ellman’s reagent to produce a chromophore that absorbs light at 412 nm. The catalytic parameters estimated through the SAT-coupled assay are fully consistent with those obtained with the published methods, but the new assay exhibits several advantages. Particularly, it depletes L-serine, thus allowing more prolonged linearity in the kinetics. Moreover, as the SAT-coupled assay does not rely on phosphate detection, it can be used to investigate the inhibitory effect of phosphate on PSP.

A novel assay for phosphoserine phosphatase exploiting serine acetyltransferase as the coupling enzyme / Marchesani, F.; Zangelmi, E.; Bruno, S.; Bettati, S.; Peracchi, A.; Campanini, B.. - In: LIFE. - ISSN 2075-1729. - 11:6(2021), pp. 485.1-485.13. [10.3390/life11060485]

A novel assay for phosphoserine phosphatase exploiting serine acetyltransferase as the coupling enzyme

Marchesani F.;Zangelmi E.;Bruno S.;Bettati S.;Peracchi A.;Campanini B.
2021

Abstract

Phosphoserine phosphatase (PSP) catalyzes the final step of de novo L-serine biosynthesis— the hydrolysis of phosphoserine to serine and inorganic phosphate—in humans, bacteria, and plants. In published works, the reaction is typically monitored through the discontinuous malachite green phosphate assay or, more rarely, through a continuous assay that couples phosphate release to the phosphorolysis of a chromogenic nucleoside by the enzyme purine nucleoside phosphorylase (PNP). These assays suffer from numerous drawbacks, and both rely on the detection of phosphate. We describe a new continuous assay that monitors the release of serine by exploiting bacterial serine acetyltransferase (SAT) as a reporter enzyme. SAT acetylates serine, consuming acetyl-CoA and releasing CoA-SH. CoA-SH spontaneously reacts with Ellman’s reagent to produce a chromophore that absorbs light at 412 nm. The catalytic parameters estimated through the SAT-coupled assay are fully consistent with those obtained with the published methods, but the new assay exhibits several advantages. Particularly, it depletes L-serine, thus allowing more prolonged linearity in the kinetics. Moreover, as the SAT-coupled assay does not rely on phosphate detection, it can be used to investigate the inhibitory effect of phosphate on PSP.
A novel assay for phosphoserine phosphatase exploiting serine acetyltransferase as the coupling enzyme / Marchesani, F.; Zangelmi, E.; Bruno, S.; Bettati, S.; Peracchi, A.; Campanini, B.. - In: LIFE. - ISSN 2075-1729. - 11:6(2021), pp. 485.1-485.13. [10.3390/life11060485]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2898715
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