Whole metagenomic shotgun (WMS) sequencing has dramatically enhanced our ability to study microbial genomics. The possibility to unveil the genetic makeup of bacteria that cannot be easily isolated has significantly expanded our microbiological horizon. Here, we report an approach aimed at uncovering novel bacterial species by the use of targeted WMS sequencing. Employing in silico data retrieved from metabolic modelling to formulate a chemically defined medium (CDM), we were able to isolate and subsequently sequence the genomes of six putative novel species of bacteria from the gut of non-human primates.
Genetic insights into the dark matter of the mammalian gut microbiota through targeted genome reconstruction / Lugli, G. A.; Alessandri, G.; Milani, C.; Viappiani, A.; Fontana, F.; Tarracchini, C.; Mancabelli, L.; Argentini, C.; Ruiz, L.; Margolles, A.; van Sinderen, D.; Turroni, F.; Ventura, M.. - In: ENVIRONMENTAL MICROBIOLOGY. - ISSN 1462-2912. - 23:6(2021), pp. 3294-3305-3305. [10.1111/1462-2920.15559]
Genetic insights into the dark matter of the mammalian gut microbiota through targeted genome reconstruction
Lugli G. A.;Alessandri G.;Milani C.;Viappiani A.;Fontana F.;Tarracchini C.;Mancabelli L.;Argentini C.;van Sinderen D.;Turroni F.;Ventura M.
2021-01-01
Abstract
Whole metagenomic shotgun (WMS) sequencing has dramatically enhanced our ability to study microbial genomics. The possibility to unveil the genetic makeup of bacteria that cannot be easily isolated has significantly expanded our microbiological horizon. Here, we report an approach aimed at uncovering novel bacterial species by the use of targeted WMS sequencing. Employing in silico data retrieved from metabolic modelling to formulate a chemically defined medium (CDM), we were able to isolate and subsequently sequence the genomes of six putative novel species of bacteria from the gut of non-human primates.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
