An amphiphilic calixarene, alone or complexed with an axle to form a pseudo-rotaxane, has been embedded into liposomes prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and the permeability of the membrane-doped liposomes towards Cl- ions has been evaluated by using lucigenin as the fluorescent probe. The pseudo-rotaxane promotes transmembrane transport of Cl- ions more than calixarene does. Surprisingly, the quenching of lucigenin was very fast for liposomes doped with the positively charged axle alone. Molecular dynamics (MD) simulations and quantum-chemical calculations were also carried out for providing a semi-quantitative support to the experimental results.
Calixarene-based artificial ionophores for chloride transport across natural liposomal bilayer: Synthesis, structure-function relationships, and computational study / Pilato, Serena; Aschi, Massimiliano; Bazzoni, Margherita; Bonati, Federica Cester; Cera, Gianpiero; Moffa, Samanta; Canale, Valentino; Ciulla, Michele; Secchi, Andrea; Arduini, Arturo; Fontana, Antonella; Siani, Gabriella. - In: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. - ISSN 0005-2736. - 1863:10(2021), p. 183667. [10.1016/j.bbamem.2021.183667]
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