In order to compar two commercially available immunoseparation/immunoconcentration methods for the isolation of Escherichia coli O157:H7 from food enrichments, a total of 80 dairy samples (40 bovine raw milk samples and 40 unpasteurized cream samples) were tested. The samples were collected in the Parmesancheese area, Parma province (northern Italy), from 9 cheese factories. The IMS requires a 24 h enrichment in m-TSB+acriflavin and after immunoseparation the Dynabeads®-bacteria complexes are plated onto CT-SMACTheVIDAS-ICE method needs a double enrichment, 6 h in m-TSB+acriflavin followed by 18 h in CT-MacConkey broth, and after automated immunoconcentration the samples are streaked onto O157:H7 ID chromogen medium. Even if no colonies of E. coli O157:H7 were isolated from the dairy samples, the IMS technique was laborious compared to the VIDAS ICE method. Moreover, confirmation of suspect colonies grown on CT-SMAC was time-consuming, due to the higher number of sorbitol-negative colonies to be tested compared to the lower number of green colonies grown on O157:H7 ID (433 vs. 153).

Comparison of the immunomagnetic separation method (IMS) with an automated immunoconcentration (VIDAS-ICE) system for detecting Escherichia coli O157:H7 in bovine raw milk and unpasteurized cream / Bonardi, S.; Chiavaro, E.; Maggi, E.. - In: INDUSTRIE ALIMENTARI. - ISSN 0019-901X. - 39:396(2000), pp. 1123-1126.

Comparison of the immunomagnetic separation method (IMS) with an automated immunoconcentration (VIDAS-ICE) system for detecting Escherichia coli O157:H7 in bovine raw milk and unpasteurized cream

Bonardi S.;Chiavaro E.;Maggi E.
2000

Abstract

In order to compar two commercially available immunoseparation/immunoconcentration methods for the isolation of Escherichia coli O157:H7 from food enrichments, a total of 80 dairy samples (40 bovine raw milk samples and 40 unpasteurized cream samples) were tested. The samples were collected in the Parmesancheese area, Parma province (northern Italy), from 9 cheese factories. The IMS requires a 24 h enrichment in m-TSB+acriflavin and after immunoseparation the Dynabeads®-bacteria complexes are plated onto CT-SMACTheVIDAS-ICE method needs a double enrichment, 6 h in m-TSB+acriflavin followed by 18 h in CT-MacConkey broth, and after automated immunoconcentration the samples are streaked onto O157:H7 ID chromogen medium. Even if no colonies of E. coli O157:H7 were isolated from the dairy samples, the IMS technique was laborious compared to the VIDAS ICE method. Moreover, confirmation of suspect colonies grown on CT-SMAC was time-consuming, due to the higher number of sorbitol-negative colonies to be tested compared to the lower number of green colonies grown on O157:H7 ID (433 vs. 153).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2885558
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