An improvement of the protocol for haploid induction through anther culture of Citrus clementina Hort. ex Tan. cv. Nules was achieved following the evaluation of a number of the factors affecting androgenesis. The influence of thidiazuron (TDZ) and three temperature pre-treatments (4°C, 25°C, 32°C) on the floral buds with respect to anther culture of C. clementina Hort. ex Tan., cv. Nules was investigated. An increased embryoid production was induced in the medium supplemented with TDZ. Pre-treatment temperatures of 4°C and 25°C were more favorable for embryo production than 32°C. Regeneration of androgenic haploid plantlets from cv. SRA 63 of C. clementina is reported here for the first time.

Improvement of Citrus clementina Hort. ex Tan. microspore-derived embryoid induction and regeneration / Germana, M. A.; Chiancone, B.. - In: PLANT CELL REPORTS. - ISSN 0721-7714. - 22:3(2003), pp. 181-187. [10.1007/s00299-003-0669-7]

Improvement of Citrus clementina Hort. ex Tan. microspore-derived embryoid induction and regeneration

Chiancone B.
2003

Abstract

An improvement of the protocol for haploid induction through anther culture of Citrus clementina Hort. ex Tan. cv. Nules was achieved following the evaluation of a number of the factors affecting androgenesis. The influence of thidiazuron (TDZ) and three temperature pre-treatments (4°C, 25°C, 32°C) on the floral buds with respect to anther culture of C. clementina Hort. ex Tan., cv. Nules was investigated. An increased embryoid production was induced in the medium supplemented with TDZ. Pre-treatment temperatures of 4°C and 25°C were more favorable for embryo production than 32°C. Regeneration of androgenic haploid plantlets from cv. SRA 63 of C. clementina is reported here for the first time.
Improvement of Citrus clementina Hort. ex Tan. microspore-derived embryoid induction and regeneration / Germana, M. A.; Chiancone, B.. - In: PLANT CELL REPORTS. - ISSN 0721-7714. - 22:3(2003), pp. 181-187. [10.1007/s00299-003-0669-7]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2884111
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