Micro-CT imaging could be considered a powerful non-invasive tool for accessing pulmonary fibrosis in mice. However, the choice of the anesthesia protocol plays a fundamental role to obtain robust and reproducible data, avoiding misinterpretations of the results. Inhaled anesthesia is commonly used for micro-CT lung imaging, but sometimes the standardization of the protocol may be challenging for routine activities in drug discovery. In this study we used micro-CT to evaluate the effects of two anesthetic protocols, consisting in Alfaxalone and Dexmedetomidine mixture, as injectable agents, and gaseous isoflurane, on vehicle and bleomycin-treated mice. No significant differences were highlighted between the protocols either for lung aeration degrees by micro-CT or histologic analyses in both the controls and bleomycin-treated groups. Our results support Alfaxalone and Dexmedetomidine mixture as a suitable and safe alternative compared to isoflurane for lung imaging. We also concluded that this injectable mixture may be applied for several imaging technologies and on different mice models.

Alfaxalone and Dexmedetomidine as an alternative to gas anesthesia for micro-CT lung imaging in a bleomycin-induced pulmonary fibrosis murine model / Ferrini, Erica; Mecozzi, Laura; Corsi, Luisa; Ragionieri, Luisa; Donofrio, Gaetano; Stellari Franco, Fabio. - In: FRONTIERS IN VETERINARY SCIENCE. - ISSN 2297-1769. - 7(2020), pp. 1-8. [10.3389/fvets.2020.588592]

Alfaxalone and Dexmedetomidine as an alternative to gas anesthesia for micro-CT lung imaging in a bleomycin-induced pulmonary fibrosis murine model

Ferrini Erica;Mecozzi Laura;Ragionieri Luisa;Donofrio Gaetano;
2020

Abstract

Micro-CT imaging could be considered a powerful non-invasive tool for accessing pulmonary fibrosis in mice. However, the choice of the anesthesia protocol plays a fundamental role to obtain robust and reproducible data, avoiding misinterpretations of the results. Inhaled anesthesia is commonly used for micro-CT lung imaging, but sometimes the standardization of the protocol may be challenging for routine activities in drug discovery. In this study we used micro-CT to evaluate the effects of two anesthetic protocols, consisting in Alfaxalone and Dexmedetomidine mixture, as injectable agents, and gaseous isoflurane, on vehicle and bleomycin-treated mice. No significant differences were highlighted between the protocols either for lung aeration degrees by micro-CT or histologic analyses in both the controls and bleomycin-treated groups. Our results support Alfaxalone and Dexmedetomidine mixture as a suitable and safe alternative compared to isoflurane for lung imaging. We also concluded that this injectable mixture may be applied for several imaging technologies and on different mice models.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2880702
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