Zearalenone (ZEN) is a Fusarium-derived xenoestrogenic mycotoxin. In plants, zearalenone-14-O-β-d-glucoside (Z14G) is the major conjugated metabolite of ZEN, and is a masked mycotoxin. Masked mycotoxins are plant-modified derivatives, which are not routinely screened in food and feed samples. Cyclodextrins (CDs) are cyclic oligosaccharides built up from D-glucopyranose units. CDs can form stable host–guest type complexes with lipophilic molecules (e.g., with some mycotoxins). In this study, the interaction of Z14G with native and chemically modified β- and γ-CDs was examined employing fluorescence spectroscopy and molecular modeling. Furthermore, the removal of Z14G from aqueous solution by insoluble β-CD bead polymer (BBP) was also tested. Our results demonstrate that Z14G forms the most stable complexes with γ-CDs under acidic and neutral conditions (K ≈ 103 L/mol). Among the CDs tested, randomly methylated γ-CD induced the highest increase in the fluorescence of Z14G (7.1-fold) and formed the most stable complexes with the mycotoxin (K = 2 × 103 L/mol). Furthermore, BBP considerably reduced the Z14G content of aqueous solution. Based on these observations, CD technology seems a promising tool to improve the fluorescence analytical detection of Z14G and to discover new mycotoxin binders which can also remove masked mycotoxins (e.g., Z14G).

Cyclodextrins can entrap zearalenone-14-glucoside: Interaction of the masked mycotoxin with cyclodextrins and cyclodextrin bead polymer / Faisal, Z.; Fliszar-Nyul, E.; Dellafiora, L.; Galaverna, G.; Dall'Asta, C.; Lemli, B.; Kunsagi-Mate, S.; Szente, L.; Poor, M.. - In: BIOMOLECULES. - ISSN 2218-273X. - 9:8(2019), p. 354. [10.3390/biom9080354]

Cyclodextrins can entrap zearalenone-14-glucoside: Interaction of the masked mycotoxin with cyclodextrins and cyclodextrin bead polymer

Dellafiora L.;Galaverna G.;Dall'Asta C.;
2019-01-01

Abstract

Zearalenone (ZEN) is a Fusarium-derived xenoestrogenic mycotoxin. In plants, zearalenone-14-O-β-d-glucoside (Z14G) is the major conjugated metabolite of ZEN, and is a masked mycotoxin. Masked mycotoxins are plant-modified derivatives, which are not routinely screened in food and feed samples. Cyclodextrins (CDs) are cyclic oligosaccharides built up from D-glucopyranose units. CDs can form stable host–guest type complexes with lipophilic molecules (e.g., with some mycotoxins). In this study, the interaction of Z14G with native and chemically modified β- and γ-CDs was examined employing fluorescence spectroscopy and molecular modeling. Furthermore, the removal of Z14G from aqueous solution by insoluble β-CD bead polymer (BBP) was also tested. Our results demonstrate that Z14G forms the most stable complexes with γ-CDs under acidic and neutral conditions (K ≈ 103 L/mol). Among the CDs tested, randomly methylated γ-CD induced the highest increase in the fluorescence of Z14G (7.1-fold) and formed the most stable complexes with the mycotoxin (K = 2 × 103 L/mol). Furthermore, BBP considerably reduced the Z14G content of aqueous solution. Based on these observations, CD technology seems a promising tool to improve the fluorescence analytical detection of Z14G and to discover new mycotoxin binders which can also remove masked mycotoxins (e.g., Z14G).
2019
Cyclodextrins can entrap zearalenone-14-glucoside: Interaction of the masked mycotoxin with cyclodextrins and cyclodextrin bead polymer / Faisal, Z.; Fliszar-Nyul, E.; Dellafiora, L.; Galaverna, G.; Dall'Asta, C.; Lemli, B.; Kunsagi-Mate, S.; Szente, L.; Poor, M.. - In: BIOMOLECULES. - ISSN 2218-273X. - 9:8(2019), p. 354. [10.3390/biom9080354]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2869857
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