A new compact diagnostic device exploiting the integration of screen printed electrode-based immunosensors and remote-controlled IoT-WiFi acquisition board has been realized and validated for diagnosis of Celiac Disease as case of study. The immunodevice is based on chemisorption of open tissue transglutaminase enzyme on the surface of gold nanoparticles-functionalized carbon screen printed electrodes. IgA and IgG anti-tissue transglutaminase target antibodies are recognized by the immobilized bioreceptor as highly specific biomarkers related to Celiac Disease. The signal from the amperometric sensor is acquired and processed through on-purpose developed IoT-WiFi integrated board, allowing for real-time data sharing on cloud services to directly notify all users (physicians, caregivers, etc.) on device outcome. The proposed solution does not require customized hardware or software. The analytical performances of the immunosensors were optimized by experimental design, obtaining diagnostically useful limit of detection (LOD) and limit of quantitation (LOQ) values (LODIgA=3.2 AU mL−1; LODIgG=1.4 AU mL−1; LOQIgA=4.6 AU mL−1; LOQIgG =2.3 AU mL−1) as well as good intermediate precision (RSD < 5%). The high discrimination capability of the IoT-Wi-Fi device between positive and negative serum control resulted to be suitable for diagnostic purposes, with outstanding statistical significance (p < 0,001)

An integrated IoT-Wi-Fi board for remote data acquisition and sharing from innovative immunosensors. Case of study: Diagnosis of celiac disease / Giannetto, Marco; Bianchi, Valentina; Gentili, Silvia; Fortunati, Simone; DE MUNARI, Ilaria; Careri, Maria. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 273:(2018), pp. 1395-1403. [10.1016/j.snb.2018.07.056]

An integrated IoT-Wi-Fi board for remote data acquisition and sharing from innovative immunosensors. Case of study: Diagnosis of celiac disease

Giannetto Marco
Writing – Original Draft Preparation
;
Bianchi Valentina
Writing – Original Draft Preparation
;
Gentili Silvia
Membro del Collaboration Group
;
Fortunati Simone
Membro del Collaboration Group
;
De Munari ilaria
Supervision
;
Careri Maria
Supervision
2018

Abstract

A new compact diagnostic device exploiting the integration of screen printed electrode-based immunosensors and remote-controlled IoT-WiFi acquisition board has been realized and validated for diagnosis of Celiac Disease as case of study. The immunodevice is based on chemisorption of open tissue transglutaminase enzyme on the surface of gold nanoparticles-functionalized carbon screen printed electrodes. IgA and IgG anti-tissue transglutaminase target antibodies are recognized by the immobilized bioreceptor as highly specific biomarkers related to Celiac Disease. The signal from the amperometric sensor is acquired and processed through on-purpose developed IoT-WiFi integrated board, allowing for real-time data sharing on cloud services to directly notify all users (physicians, caregivers, etc.) on device outcome. The proposed solution does not require customized hardware or software. The analytical performances of the immunosensors were optimized by experimental design, obtaining diagnostically useful limit of detection (LOD) and limit of quantitation (LOQ) values (LODIgA=3.2 AU mL−1; LODIgG=1.4 AU mL−1; LOQIgA=4.6 AU mL−1; LOQIgG =2.3 AU mL−1) as well as good intermediate precision (RSD < 5%). The high discrimination capability of the IoT-Wi-Fi device between positive and negative serum control resulted to be suitable for diagnostic purposes, with outstanding statistical significance (p < 0,001)
An integrated IoT-Wi-Fi board for remote data acquisition and sharing from innovative immunosensors. Case of study: Diagnosis of celiac disease / Giannetto, Marco; Bianchi, Valentina; Gentili, Silvia; Fortunati, Simone; DE MUNARI, Ilaria; Careri, Maria. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 273:(2018), pp. 1395-1403. [10.1016/j.snb.2018.07.056]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2848595
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