Mycotoxins are secondary metabolites of fungi that contaminate food and feed, and are involved in a series of foodborne illnesses and disorders in humans and animals. The mitigation of mycotoxin content via enzymatic degradation is a strategy to ensure safer food and feed, and to addresstheforthcomingissuesinviewoftheglobaltradeandsustainability. Nevertheless, the search for active enzymes is still challenging and time-consuming. The in silico analysis may strongly support the research by providing the evidence-based hierarchization of enzymes for a rational designofmoreeffectiveexperimentaltrials. Thepresentworkdealtwiththedegradationofaflatoxin B1 andM1 bylaccaseenzymesfromTrametesversicolor. Theenzymes–substrateinteractionforvarious enzymeisoformswasinvestigatedthrough3Dmolecularmodelingtechniques. Structuraldifferences among the isoforms have been pinpointed, which may cause different patterns of interaction between aflatoxin B1 and M1. The possible formation of different products of degradation can be argued accordingly. Moreover, the laccase gamma isoform was identified as the most suitable for protein engineering aimed at ameliorating the substrate specificity. Overall, 3D modeling proved to be an effective analytical tool to assess the enzyme–substrate interaction and provided a solid foothold for supporting the search of degrading enzyme at the early stage

Degradation of aflatoxins by means of laccases from trametes versicolor: An in silico insight / Dellafiora, Luca; Galaverna, Gianni; Reverberi, M.; Dall'Asta, Chiara. - In: TOXINS. - ISSN 2072-6651. - 9:1(2017). [10.3390/toxins9010017]

Degradation of aflatoxins by means of laccases from trametes versicolor: An in silico insight

Dellafiora L.;Galaverna G.;Dall’asta C.
2017

Abstract

Mycotoxins are secondary metabolites of fungi that contaminate food and feed, and are involved in a series of foodborne illnesses and disorders in humans and animals. The mitigation of mycotoxin content via enzymatic degradation is a strategy to ensure safer food and feed, and to addresstheforthcomingissuesinviewoftheglobaltradeandsustainability. Nevertheless, the search for active enzymes is still challenging and time-consuming. The in silico analysis may strongly support the research by providing the evidence-based hierarchization of enzymes for a rational designofmoreeffectiveexperimentaltrials. Thepresentworkdealtwiththedegradationofaflatoxin B1 andM1 bylaccaseenzymesfromTrametesversicolor. Theenzymes–substrateinteractionforvarious enzymeisoformswasinvestigatedthrough3Dmolecularmodelingtechniques. Structuraldifferences among the isoforms have been pinpointed, which may cause different patterns of interaction between aflatoxin B1 and M1. The possible formation of different products of degradation can be argued accordingly. Moreover, the laccase gamma isoform was identified as the most suitable for protein engineering aimed at ameliorating the substrate specificity. Overall, 3D modeling proved to be an effective analytical tool to assess the enzyme–substrate interaction and provided a solid foothold for supporting the search of degrading enzyme at the early stage
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2835421
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 25
  • ???jsp.display-item.citation.isi??? 21
social impact