Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.

Isolation and characterization of human lung lymphatic endothelial cells / Lorusso, Bruno; Falco, Angela; Madeddu, Denise; Frati, Caterina; Cavalli, Stefano; Graiani, Gallia; Gervasi, Andrea; Rinaldi, Laura; Lagrasta, Costanza; Maselli, Davide; Gnetti, Letizia; Silini, Enrico Maria; Quaini, Eugenio; Ampollini, Luca; Carbognani, Paolo; Quaini, Federico. - In: BIOMED RESEARCH INTERNATIONAL. - ISSN 2314-6133. - 2015:(2015), pp. 747864-747875. [10.1155/2015/747864]

Isolation and characterization of human lung lymphatic endothelial cells

LORUSSO, BRUNO;FALCO, Angela;MADEDDU, Denise;FRATI, Caterina;CAVALLI, Stefano;GRAIANI, Gallia;GERVASI, Andrea;RINALDI, LAURA;SILINI, Enrico Maria;AMPOLLINI, Luca;CARBOGNANI, Paolo;QUAINI, Federico
2015-01-01

Abstract

Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.
2015
Isolation and characterization of human lung lymphatic endothelial cells / Lorusso, Bruno; Falco, Angela; Madeddu, Denise; Frati, Caterina; Cavalli, Stefano; Graiani, Gallia; Gervasi, Andrea; Rinaldi, Laura; Lagrasta, Costanza; Maselli, Davide; Gnetti, Letizia; Silini, Enrico Maria; Quaini, Eugenio; Ampollini, Luca; Carbognani, Paolo; Quaini, Federico. - In: BIOMED RESEARCH INTERNATIONAL. - ISSN 2314-6133. - 2015:(2015), pp. 747864-747875. [10.1155/2015/747864]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2815980
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