Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.
Isolation and characterization of human lung lymphatic endothelial cells / Lorusso, B., Falco, A., Madeddu, D., Frati, C., Cavalli, S., Graiani, G., Gervasi, A., Rinaldi, L., Lagrasta, C., Maselli, D., Gnetti, L., Silini, E.M., Quaini, E., Ampollini, L., Carbognani, P., Quaini, F.. - In: BIOMED RESEARCH INTERNATIONAL. - ISSN 2314-6133. - 2015:(2015), pp. 747864-747875. [10.1155/2015/747864]
Isolation and characterization of human lung lymphatic endothelial cells
LORUSSO, BRUNO;FALCO, Angela;MADEDDU, Denise;FRATI, Caterina;CAVALLI, Stefano;GRAIANI, Gallia;GERVASI, Andrea;RINALDI, LAURA;SILINI, Enrico Maria;AMPOLLINI, Luca;CARBOGNANI, Paolo;QUAINI, Federico
2015-01-01
Abstract
Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
