Residues 11 to 27 of the hepatitis B virus nucleocapsid antigen contain a cytotoxic T-cell epitope that is recognized by cytotoxic T cells from virtually all HLA-A2-positive patients with acute hepatitis B virus infection. Using panels of truncated and overlapping peptides, we now show that the optimal amino acid sequence recognized by cytotoxic T cells is a 10-mer (residues 18 to 27) containing the predicted peptide-binding motif for HLA-A2 and that this peptide can stimulate cytotoxic T cells able to recognize endogenously synthesized hepatitis B core antigen. Since patients with chronic hepatitis B virus infection fail to mount an efficient cytotoxic T-cell response to it, this epitope might serve as the starting point for the design of synthetic peptide-based immunotherapeutic strategies to terminate persistent viral infection.

Definition of a minimal optimal cytotoxic T-cell epitope within the hepatitis B virus nucleocapsid protein / Bertoletti, A; Chisari, F. V; Penna, A; Guilhot, S; Galati, L; Missale, G; Fowler, P; Schlicht, H. J; Vitiello, A; Chesnut, R. C; Ferrari, Carlo. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 67:4(1993), p. 2376-80.

Definition of a minimal optimal cytotoxic T-cell epitope within the hepatitis B virus nucleocapsid protein

Missale, G;FERRARI, Carlo
1993-01-01

Abstract

Residues 11 to 27 of the hepatitis B virus nucleocapsid antigen contain a cytotoxic T-cell epitope that is recognized by cytotoxic T cells from virtually all HLA-A2-positive patients with acute hepatitis B virus infection. Using panels of truncated and overlapping peptides, we now show that the optimal amino acid sequence recognized by cytotoxic T cells is a 10-mer (residues 18 to 27) containing the predicted peptide-binding motif for HLA-A2 and that this peptide can stimulate cytotoxic T cells able to recognize endogenously synthesized hepatitis B core antigen. Since patients with chronic hepatitis B virus infection fail to mount an efficient cytotoxic T-cell response to it, this epitope might serve as the starting point for the design of synthetic peptide-based immunotherapeutic strategies to terminate persistent viral infection.
Definition of a minimal optimal cytotoxic T-cell epitope within the hepatitis B virus nucleocapsid protein / Bertoletti, A; Chisari, F. V; Penna, A; Guilhot, S; Galati, L; Missale, G; Fowler, P; Schlicht, H. J; Vitiello, A; Chesnut, R. C; Ferrari, Carlo. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 67:4(1993), p. 2376-80.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2814020
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