Using a combination of molecular modeling and spectroscopic experiments, the naturally occurring, pharmacologically active hypericin compound is shown to form a stable complex with the dimeric form of β-lactoglobulin (β-LG). Binding is predicted to occur at the narrowest cleft found at the interface between monomers in the dimeric β-LG. The complex is able to preserve the fluorescence and singlet oxygen photosensitizing properties of the dye. The equilibrium constant for hypericin binding has been determined as Ka = 1.40 ± 0.07 μM−1, equivalent to a dissociation constant, Kd = 0.71 ± 0.03 μM. The complex is active against Staphylococcus aureus bacteria. Overall, the results are encouraging for pursuing the potential application of the complex between hypericin and β-LG as a nanodevice with bactericidal properties for disinfection.
The complex of hypericin with β-lactoglobulin has antimicrobial activity with perspective applications in dairy industry / Beatriz Rodríguez Amigo, ; Delcanale, Pietro; Gabriel, Rotger; Jordi Juárez Jiménez, ; Abbruzzetti, Stefania; Summer, Andrea; Montserrat, Agut; Javier Luque, F.; Santi, Nonell; Viappiani, Cristiano. - In: JOURNAL OF DAIRY SCIENCE. - ISSN 1525-3198. - 98:(2015), pp. 89-94. [10.3168/jds.2014-8691]
The complex of hypericin with β-lactoglobulin has antimicrobial activity with perspective applications in dairy industry
Pietro Delcanale;ABBRUZZETTI, Stefania;SUMMER, Andrea;VIAPPIANI, Cristiano
2015-01-01
Abstract
Using a combination of molecular modeling and spectroscopic experiments, the naturally occurring, pharmacologically active hypericin compound is shown to form a stable complex with the dimeric form of β-lactoglobulin (β-LG). Binding is predicted to occur at the narrowest cleft found at the interface between monomers in the dimeric β-LG. The complex is able to preserve the fluorescence and singlet oxygen photosensitizing properties of the dye. The equilibrium constant for hypericin binding has been determined as Ka = 1.40 ± 0.07 μM−1, equivalent to a dissociation constant, Kd = 0.71 ± 0.03 μM. The complex is active against Staphylococcus aureus bacteria. Overall, the results are encouraging for pursuing the potential application of the complex between hypericin and β-LG as a nanodevice with bactericidal properties for disinfection.File | Dimensione | Formato | |
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