Although the assessment of α -amylase is an essential part of the diagnostic workout of several pancreatic and extra-pancreatic disorders, its enzymatic activity is significantly reduced in the presence of cell-free hemoglobin such as in samples with purious hemolysis, due to chemical and spectrophotometric interference. We developed a new reagent that provides reliable results on hemolyzed biological specimens.
Development of a novel, hemolysis-resistant reagent for assessment of α-amylase in biological fluids / Ronda, Luca; Mozzarelli, Andrea; Aloe, R; Lippi, G.. - In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE. - ISSN 1434-6621. - 15:(2013), pp. 1409-1415. [10.1515/cclm-2012-0376]
Development of a novel, hemolysis-resistant reagent for assessment of α-amylase in biological fluids
RONDA, Luca;MOZZARELLI, Andrea;
2013-01-01
Abstract
Although the assessment of α -amylase is an essential part of the diagnostic workout of several pancreatic and extra-pancreatic disorders, its enzymatic activity is significantly reduced in the presence of cell-free hemoglobin such as in samples with purious hemolysis, due to chemical and spectrophotometric interference. We developed a new reagent that provides reliable results on hemolyzed biological specimens.| File | Dimensione | Formato | |
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