Although adoptive transfer of donor lymphocytes protects from infections and relapse after allogeneic hematopoietic stem cell transplantation in both mice and in men, it is associated with a high risk of graft versus host disease (GvHD) which rises with HLA mismatching and the number of T lymphocytes that are infused. Elimination/reduction of alloreactive donor T lymphocytes is an appealing approach and several strategies have been proposed. Here we describe generation of anti-3rd party T lymphocytes under conditions of IL-2 deprivation and their effects in a pre-clinical murine model. Our results clearly indicated that anti-3rd party T lymphocytes generated on a large scale by means of IL-2 deprivation maintain a broad T cell repertoire, do not proliferate in a mixed lymphocyte reaction and do not cause GvHD in NOD-SCID mice. These anti-3rd party lymphocytes contain a large adaptive T regulatory cell subset which might contribute to in vitro and in vivo immune modulation.

Large-scale generation of human allodepleted anti-3rd party lymphocytes / M., De Ioanni; M., Di Ianni; E., Bonifacio; L., Moretti; D., Cecchini; F., Bazzucchi; A., Terenzi; T., Aloisi; F., Falzetti; Aversa, Franco; Y., Reisner; Martelli, M. F.; A., Tabilio. - In: BLOOD CELLS, MOLECULES, & DISEASES. - ISSN 1079-9796. - 40:(2008), pp. 106-112.

Large-scale generation of human allodepleted anti-3rd party lymphocytes.

AVERSA, Franco;
2008-01-01

Abstract

Although adoptive transfer of donor lymphocytes protects from infections and relapse after allogeneic hematopoietic stem cell transplantation in both mice and in men, it is associated with a high risk of graft versus host disease (GvHD) which rises with HLA mismatching and the number of T lymphocytes that are infused. Elimination/reduction of alloreactive donor T lymphocytes is an appealing approach and several strategies have been proposed. Here we describe generation of anti-3rd party T lymphocytes under conditions of IL-2 deprivation and their effects in a pre-clinical murine model. Our results clearly indicated that anti-3rd party T lymphocytes generated on a large scale by means of IL-2 deprivation maintain a broad T cell repertoire, do not proliferate in a mixed lymphocyte reaction and do not cause GvHD in NOD-SCID mice. These anti-3rd party lymphocytes contain a large adaptive T regulatory cell subset which might contribute to in vitro and in vivo immune modulation.
2008
Large-scale generation of human allodepleted anti-3rd party lymphocytes / M., De Ioanni; M., Di Ianni; E., Bonifacio; L., Moretti; D., Cecchini; F., Bazzucchi; A., Terenzi; T., Aloisi; F., Falzetti; Aversa, Franco; Y., Reisner; Martelli, M. F.; A., Tabilio. - In: BLOOD CELLS, MOLECULES, & DISEASES. - ISSN 1079-9796. - 40:(2008), pp. 106-112.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2438674
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