A 10-mer duplex formed between a PNA containing a `chiral box' of three adjacent d-Lys-based monomers and its complementary DNA strand has been crystallized for the first time. Crystals have been obtained using PEG 8000 as precipitant and cacodylate at pH 6.3 as buffer. The crystals belong to the space group P31 or to its enantiomorph P32, with unit-cell parameters a = b = 35.00, c = 35.91 Å. A complete data set has been collected at the synchrotron source Elettra in Trieste to 1.85 Å resolution, using a single frozen crystal.

Crystallization and preliminary X-ray diffraction studies of a D-lysine-based chiral PNA–DNA duplex / Valeria Menchise;Giuseppina De Simone;Roberto Corradini;Stefano Sforza;Nicola Sorrentino;Alessandra Romanelli;Michele Saviano;Carlo Pedone. - In: ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY. - ISSN 0907-4449. - 58(2002), pp. 553-555. [10.1107/S0907444902001427]

Crystallization and preliminary X-ray diffraction studies of a D-lysine-based chiral PNA–DNA duplex

CORRADINI, Roberto;SFORZA, Stefano;
2002

Abstract

A 10-mer duplex formed between a PNA containing a `chiral box' of three adjacent d-Lys-based monomers and its complementary DNA strand has been crystallized for the first time. Crystals have been obtained using PEG 8000 as precipitant and cacodylate at pH 6.3 as buffer. The crystals belong to the space group P31 or to its enantiomorph P32, with unit-cell parameters a = b = 35.00, c = 35.91 Å. A complete data set has been collected at the synchrotron source Elettra in Trieste to 1.85 Å resolution, using a single frozen crystal.
File in questo prodotto:
File Dimensione Formato  
Acta_Cryst_D_2002.pdf

non disponibili

Tipologia: Abstract
Licenza: Creative commons
Dimensione 216.18 kB
Formato Adobe PDF
216.18 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2436360
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 8
social impact