Aiming at the rational development of genetically-encoded photosensitisers, the production of singlet oxygen has been assessed for a number of class-2 Green Fluorescent Protein (GFP) mutants by means of time-resolved near-infrared luminescence detection. The accessibility of molecular oxygen to the chromophore seems to play a major role in the ability of GFPs to photosensitise singlet oxygen and this can be modulated by introducing specific mutations such as replacement of His148 by a less bulky amino acid. GFPs are also good singlet oxygen quenchers, hence further developments in this area should also seek to eliminate those amino acids with the highest quenching ability, particularly those at the protein surface and in the vicinity of the chromophore.

Singlet oxygen photosensitisation by GFP mutants: Oxygen accessibility to the chromophore / A., Jiménez Banzo; X., Ragàs; Abbruzzetti, Stefania; Viappiani, Cristiano; Campanini, Barbara; C., Flors; S., Nonell. - In: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES. - ISSN 1474-905X. - 9:(2010), pp. 1336-1341. [10.1039/c0pp00125b]

Singlet oxygen photosensitisation by GFP mutants: Oxygen accessibility to the chromophore

ABBRUZZETTI, Stefania;VIAPPIANI, Cristiano;CAMPANINI, Barbara;
2010

Abstract

Aiming at the rational development of genetically-encoded photosensitisers, the production of singlet oxygen has been assessed for a number of class-2 Green Fluorescent Protein (GFP) mutants by means of time-resolved near-infrared luminescence detection. The accessibility of molecular oxygen to the chromophore seems to play a major role in the ability of GFPs to photosensitise singlet oxygen and this can be modulated by introducing specific mutations such as replacement of His148 by a less bulky amino acid. GFPs are also good singlet oxygen quenchers, hence further developments in this area should also seek to eliminate those amino acids with the highest quenching ability, particularly those at the protein surface and in the vicinity of the chromophore.
Singlet oxygen photosensitisation by GFP mutants: Oxygen accessibility to the chromophore / A., Jiménez Banzo; X., Ragàs; Abbruzzetti, Stefania; Viappiani, Cristiano; Campanini, Barbara; C., Flors; S., Nonell. - In: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES. - ISSN 1474-905X. - 9:(2010), pp. 1336-1341. [10.1039/c0pp00125b]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2330643
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 31
  • ???jsp.display-item.citation.isi??? 33
social impact