The aim of this work was to develop and validate a specific reversed phase high performance liquid chromatographic method (HPLC) using diode array detection (DAD) for the determination of flavanones, which is the dominant flavonoid class in the genus citrus. The procedures and criteria used for validation of the method including sensitivity, linearity, repeatability, recovery, and robustness were carried out following the EURACHEM guidelines. The validated RP-HPLC-DAD method was applied for the determination of the mainly flavanone glycosides contained in dried blood orange peel and dried lemon peel, and for the quantitation of flavanone glycosides and hesperetin in nutraceutical products.

Determination of flavanones in citrus byproducts and nutraceutical products by a validated RP-HPLC method / Corradini, Claudio; Borromei, C.; Cavazza, Antonella; Merusi, C.; Rossi, A.; Nicoletti, I.. - In: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. - ISSN 1082-6076. - 32 (10):(2009), pp. 1448-1462. [10.1080/10826070902901374]

Determination of flavanones in citrus byproducts and nutraceutical products by a validated RP-HPLC method

CORRADINI, Claudio;CAVAZZA, Antonella;C. Merusi;
2009-01-01

Abstract

The aim of this work was to develop and validate a specific reversed phase high performance liquid chromatographic method (HPLC) using diode array detection (DAD) for the determination of flavanones, which is the dominant flavonoid class in the genus citrus. The procedures and criteria used for validation of the method including sensitivity, linearity, repeatability, recovery, and robustness were carried out following the EURACHEM guidelines. The validated RP-HPLC-DAD method was applied for the determination of the mainly flavanone glycosides contained in dried blood orange peel and dried lemon peel, and for the quantitation of flavanone glycosides and hesperetin in nutraceutical products.
2009
Determination of flavanones in citrus byproducts and nutraceutical products by a validated RP-HPLC method / Corradini, Claudio; Borromei, C.; Cavazza, Antonella; Merusi, C.; Rossi, A.; Nicoletti, I.. - In: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. - ISSN 1082-6076. - 32 (10):(2009), pp. 1448-1462. [10.1080/10826070902901374]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2309528
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