Yield and amplificability of different DNA extraction procedures for traceability in the dairy food chain.

Seven DNA extraction procedures were compared for their ability to produce good quality DNA if applied to outputs of the dairy food chain. The efficacy and the efficiency of the protocols were tested. PCR and Real-time PCR specific amplification with appropriate bovine primers were used to assess the quality of the genomic DNA extracted with the compared procedures. The results were also evaluated from the economical point of view, in order to determine which of the seven protocols was the simplest, the most reliable and the most affordable for molecular traceability within this important food chain in order to protect producers from unfair competition and consumers from frauds and adulterations.