In this study, we examined the mechanism of action of the novel epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor 5-benzylidene-hydantoin UPR1024, whose structure was designed to interact at the ATP-binding site of EGFR. The compound had antiproliferative and proapoptotic effects when tested on the non-small cell lung cancer cell line A549. The growth inhibitory effect was associated with an accumulation of the cells in the S phase of the cell cycle. Moreover, UPR1024 induced significant level of DNA strand breaks associated with increased expression of p53 and p21(WAF1) proteins, suggesting an additive mechanism of action. The presence of wild-type p53 improved the drug efficacy, although the effect was also detectable in p53 null cells. We also noted apoptotic cell death after treatment with UPR1024 at concentrations above 10 mumol/L for >24 h, with involvement of both the extrinsic and intrinsic pathways. The present data show that UPR1024 may be considered a combi-molecule capable of both blocking EGFR tyrosine kinase activity and inducing genomic DNA damage. UPR1024 or its derivatives might serve as a basis for development of drugs for the treatment of lung cancer in patients resistant to classic tyrosine kinase inhibitors.

Dual mechanisms of action of the 5-benzylidene-hydantoin UPR1024 on lung cancer cell lines / Cavazzoni, Andrea; Alfieri, Roberta; Carmi, C.; Zuliani, Valentina; Galetti, Maricla; Fumarola, Claudia; Frazzi, Raffaele; Bonelli, Mara; Bordi, Fabrizio; Lodola, Alessio; Mor, Marco; Petronini, Pier Giorgio. - In: MOLECULAR CANCER THERAPEUTICS. - ISSN 1535-7163. - 7:(2008), pp. 361-370. [10.1158/1535-7163.MCT-07-0477]

Dual mechanisms of action of the 5-benzylidene-hydantoin UPR1024 on lung cancer cell lines

CAVAZZONI, Andrea;ALFIERI, Roberta;ZULIANI, Valentina;GALETTI, Maricla;FUMAROLA, Claudia;FRAZZI, Raffaele;BONELLI, Mara;BORDI, Fabrizio;LODOLA, Alessio;MOR, Marco;PETRONINI, Pier Giorgio
2008-01-01

Abstract

In this study, we examined the mechanism of action of the novel epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor 5-benzylidene-hydantoin UPR1024, whose structure was designed to interact at the ATP-binding site of EGFR. The compound had antiproliferative and proapoptotic effects when tested on the non-small cell lung cancer cell line A549. The growth inhibitory effect was associated with an accumulation of the cells in the S phase of the cell cycle. Moreover, UPR1024 induced significant level of DNA strand breaks associated with increased expression of p53 and p21(WAF1) proteins, suggesting an additive mechanism of action. The presence of wild-type p53 improved the drug efficacy, although the effect was also detectable in p53 null cells. We also noted apoptotic cell death after treatment with UPR1024 at concentrations above 10 mumol/L for >24 h, with involvement of both the extrinsic and intrinsic pathways. The present data show that UPR1024 may be considered a combi-molecule capable of both blocking EGFR tyrosine kinase activity and inducing genomic DNA damage. UPR1024 or its derivatives might serve as a basis for development of drugs for the treatment of lung cancer in patients resistant to classic tyrosine kinase inhibitors.
2008
Dual mechanisms of action of the 5-benzylidene-hydantoin UPR1024 on lung cancer cell lines / Cavazzoni, Andrea; Alfieri, Roberta; Carmi, C.; Zuliani, Valentina; Galetti, Maricla; Fumarola, Claudia; Frazzi, Raffaele; Bonelli, Mara; Bordi, Fabrizio; Lodola, Alessio; Mor, Marco; Petronini, Pier Giorgio. - In: MOLECULAR CANCER THERAPEUTICS. - ISSN 1535-7163. - 7:(2008), pp. 361-370. [10.1158/1535-7163.MCT-07-0477]
File in questo prodotto:
File Dimensione Formato  
MolCancerThe 2008.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 300.85 kB
Formato Adobe PDF
300.85 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2294045
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 67
  • ???jsp.display-item.citation.isi??? 67
social impact