Vitamin E, known for its great nutritional importance, is normally included in animal diets as DL-tocopherol acetate. The authors propose a method that makes it possible to determine the concentration of vitamin E in plasma without saponification. This method enable to avoid aggressive treatments on the analyte and complex procedures; it detects vitamin E only in form of DL-x-tocopherol. Lipoproteins of analysed plasma were denaturised by methanol. Vitamin E was extracted by petroleum ether in presence of NaCl. The extract was dried by rotavapor at 45 °C, solubilized by methanol and injected in HPLC (C18 column, reversed phase). The quantitative determination was carried out by UV detector settled on 294 nm. Tests of repeatability inter-analysis and intra-analysis gave coefficient of variability (CV%) respectively of 1.64 and 2.41%. The mean recovery was 100%.

Simplified HPLC-UV method for the determination of alfa-tocopherol in plasma / Renzi, Marco; Righi, Federico; Quarantelli, C.; Quarantelli, Afro; Bonomi, A.. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - 4:2(2005), pp. 191-195. [doi/pdf/10.4081/ijas.2005.191]

Simplified HPLC-UV method for the determination of alfa-tocopherol in plasma.

RENZI, Marco;RIGHI, Federico;QUARANTELLI, Afro;
2005-01-01

Abstract

Vitamin E, known for its great nutritional importance, is normally included in animal diets as DL-tocopherol acetate. The authors propose a method that makes it possible to determine the concentration of vitamin E in plasma without saponification. This method enable to avoid aggressive treatments on the analyte and complex procedures; it detects vitamin E only in form of DL-x-tocopherol. Lipoproteins of analysed plasma were denaturised by methanol. Vitamin E was extracted by petroleum ether in presence of NaCl. The extract was dried by rotavapor at 45 °C, solubilized by methanol and injected in HPLC (C18 column, reversed phase). The quantitative determination was carried out by UV detector settled on 294 nm. Tests of repeatability inter-analysis and intra-analysis gave coefficient of variability (CV%) respectively of 1.64 and 2.41%. The mean recovery was 100%.
2005
Simplified HPLC-UV method for the determination of alfa-tocopherol in plasma / Renzi, Marco; Righi, Federico; Quarantelli, C.; Quarantelli, Afro; Bonomi, A.. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - 4:2(2005), pp. 191-195. [doi/pdf/10.4081/ijas.2005.191]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2294005
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