CD40 ligand (CD40L) stimulation induces proinflammatory and immunomodulatory activity in monocytes. Here, we report on the effects of the steroid hormone 1a,25-dihydroxyvitamin D3 (1,25D3) on human blood monocytes that have been stimulated with the CD40L ligand. Co-treatment of CD40L-stimulated monocytes with 1,25D3 resulted in reduced production and secretion of tumor necrosis factor (TNF)-a and interleukin (IL)-1b, as well as in reduced expression of the surface co-stimulatory molecules CD80 and CD86. In addition, costimulation of CD4+ T lymphocytes by monocytes co-treated with CD40L and 1,25D3 resulted in reduced cell proliferation and diminished interferon (IFN)-c but enhanced IL-10 production by CD4+ T cells. Finally, 1,25D3 interfered with the ability of CD40L to rescue monocytes from apoptosis induced by serum withdrawal. These findings suggest that 1,25D3 may regulate the interaction of monocytes with T cells or other cell types that express CD40L, thus influencing the outcome of the immune or inflammatory response.

1alpha,25-dihydroxyvitamin D3 inhibits CD40L-induced proinflammatoryand immunomodulatory activity in Human Monocytes / Bergamini, A; Almerighi, C; Sinistro, A; Cavazza, Antonella; Ciaprini, C; Rocchi, G.. - In: CYTOKINE. - ISSN 1043-4666. - 45:(2009), pp. 190-197. [10.1016/j.cyto.2008.12.009]

1alpha,25-dihydroxyvitamin D3 inhibits CD40L-induced proinflammatoryand immunomodulatory activity in Human Monocytes

CAVAZZA, Antonella;
2009-01-01

Abstract

CD40 ligand (CD40L) stimulation induces proinflammatory and immunomodulatory activity in monocytes. Here, we report on the effects of the steroid hormone 1a,25-dihydroxyvitamin D3 (1,25D3) on human blood monocytes that have been stimulated with the CD40L ligand. Co-treatment of CD40L-stimulated monocytes with 1,25D3 resulted in reduced production and secretion of tumor necrosis factor (TNF)-a and interleukin (IL)-1b, as well as in reduced expression of the surface co-stimulatory molecules CD80 and CD86. In addition, costimulation of CD4+ T lymphocytes by monocytes co-treated with CD40L and 1,25D3 resulted in reduced cell proliferation and diminished interferon (IFN)-c but enhanced IL-10 production by CD4+ T cells. Finally, 1,25D3 interfered with the ability of CD40L to rescue monocytes from apoptosis induced by serum withdrawal. These findings suggest that 1,25D3 may regulate the interaction of monocytes with T cells or other cell types that express CD40L, thus influencing the outcome of the immune or inflammatory response.
2009
1alpha,25-dihydroxyvitamin D3 inhibits CD40L-induced proinflammatoryand immunomodulatory activity in Human Monocytes / Bergamini, A; Almerighi, C; Sinistro, A; Cavazza, Antonella; Ciaprini, C; Rocchi, G.. - In: CYTOKINE. - ISSN 1043-4666. - 45:(2009), pp. 190-197. [10.1016/j.cyto.2008.12.009]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2283860
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