The possible degradation of the tumor antigen epitope gp100280–288 (YLEPGPVTA) in the presence of the monocyte-like line U937, and the effect of degradation on the in vitro-measured immune recognition, were investigated by chromatographic techniques and immunological assays. Results indicate a rapid hydrolysis of the substrate in the presence of the model cells, which is consistent with the hypothesis that degradation of gp100280–288 is caused by the activity of U937-expressed enzymes, specifically amino- and carboxypeptidases. On the other hand, these results do not support the involvement of other enzymes known to be expressed by U937 cells. From a functional point of view, these data indicate that the degradation of gp100280–288 severely hampered recognition by specific CTL clones. The results obtained may provide a model for epitope degradation by the antigen-presenting cells found in defined anatomical compartments and may, at least in part, account for the low activity of peptide-based antineoplastic vaccines, as well as for the transience of the effects of subcutaneously administered peptides in general.
Degradation of the immunogenic peptide gp100280-288 by the monocyte-like U937 cell line / Albo, F; Cavazza, Antonella; Giardina, B; Lippa, S; Marini, M; Roda, L. G.; Spagnoli, G.. - In: PEPTIDES. - ISSN 0196-9781. - 24:(2003), pp. 371-378. [10.1016/S0196-9781(03)00051-2]
Degradation of the immunogenic peptide gp100280-288 by the monocyte-like U937 cell line
CAVAZZA, Antonella;
2003-01-01
Abstract
The possible degradation of the tumor antigen epitope gp100280–288 (YLEPGPVTA) in the presence of the monocyte-like line U937, and the effect of degradation on the in vitro-measured immune recognition, were investigated by chromatographic techniques and immunological assays. Results indicate a rapid hydrolysis of the substrate in the presence of the model cells, which is consistent with the hypothesis that degradation of gp100280–288 is caused by the activity of U937-expressed enzymes, specifically amino- and carboxypeptidases. On the other hand, these results do not support the involvement of other enzymes known to be expressed by U937 cells. From a functional point of view, these data indicate that the degradation of gp100280–288 severely hampered recognition by specific CTL clones. The results obtained may provide a model for epitope degradation by the antigen-presenting cells found in defined anatomical compartments and may, at least in part, account for the low activity of peptide-based antineoplastic vaccines, as well as for the transience of the effects of subcutaneously administered peptides in general.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.