This study was aimed at investigating the possible involvement of the actin cytoskeleton in the modulation of host permissiveness to A/NWS/33 human influenza virus infection in two mammalian (MDCK and LLC-MK2) cell lines in vitro. During the early stages of infection, no appreciable association between incoming NWS/33 virions and cortical actin was detectable in the permissive MDCK model by confocal microscopy, while extensive colocalization and a slower infection progression were observed in LLC-MK2 cells. In the latter model, we also demonstrated the inability of the virus to carry out multiple replication cycles, irrespective of the presence of cleaved HA subunits in the released virions. Treatment with the actin-depolymerizing agent cytochalasin D significantly increased the infection efficiency in LLC-MK2 cells, while a detrimental effect was observed in the MDCK cell line. Our data suggest a selective role of the actin network in inducing a restriction to influenza virus replication, mostly depending on its molecular organization, the host cell type and virus replication phase.

Host-cell-dependent role of actin cytoskeleton during the replication of a human strain of influenza A virus / Arcangeletti, Maria Cristina; DE CONTO, Flora; Ferraglia, Francesca; Pinardi, Federica; Gatti, Rita; Orlandini, Guido; Covan, Silvia; Motta, Federica; Rodighiero, Isabella; Dettori, Giuseppe; Chezzi, Carlo. - In: ARCHIVES OF VIROLOGY. - ISSN 0304-8608. - 153(7):(2008), pp. 1209-1221. [10.1007/s00705-008-0103-0]

Host-cell-dependent role of actin cytoskeleton during the replication of a human strain of influenza A virus

ARCANGELETTI, Maria Cristina;DE CONTO, Flora;FERRAGLIA, Francesca;PINARDI, Federica;GATTI, Rita;ORLANDINI, Guido;COVAN, Silvia;MOTTA, Federica;RODIGHIERO, Isabella;DETTORI, Giuseppe;CHEZZI, Carlo
2008-01-01

Abstract

This study was aimed at investigating the possible involvement of the actin cytoskeleton in the modulation of host permissiveness to A/NWS/33 human influenza virus infection in two mammalian (MDCK and LLC-MK2) cell lines in vitro. During the early stages of infection, no appreciable association between incoming NWS/33 virions and cortical actin was detectable in the permissive MDCK model by confocal microscopy, while extensive colocalization and a slower infection progression were observed in LLC-MK2 cells. In the latter model, we also demonstrated the inability of the virus to carry out multiple replication cycles, irrespective of the presence of cleaved HA subunits in the released virions. Treatment with the actin-depolymerizing agent cytochalasin D significantly increased the infection efficiency in LLC-MK2 cells, while a detrimental effect was observed in the MDCK cell line. Our data suggest a selective role of the actin network in inducing a restriction to influenza virus replication, mostly depending on its molecular organization, the host cell type and virus replication phase.
2008
Host-cell-dependent role of actin cytoskeleton during the replication of a human strain of influenza A virus / Arcangeletti, Maria Cristina; DE CONTO, Flora; Ferraglia, Francesca; Pinardi, Federica; Gatti, Rita; Orlandini, Guido; Covan, Silvia; Motta, Federica; Rodighiero, Isabella; Dettori, Giuseppe; Chezzi, Carlo. - In: ARCHIVES OF VIROLOGY. - ISSN 0304-8608. - 153(7):(2008), pp. 1209-1221. [10.1007/s00705-008-0103-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1845888
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