Free radical production is a biological phenomenon which occurs constantly during metabolic processes. Under normal conditions, Reactive Oxygen Species production is balanced by scavenger enzymes and antioxidant molecules. An increase of oxidative stress, which has been demonstrated in dairy cattle in particular stressfull conditions such as peripartum, can be related with decreased fertility. The aim of this study was to investigate the anti-oxidative status of mares around parturition. Blood samples were taken from 17 thoroughbred mares at days –24 (T1), 1 (T2) and +9 (T3) relative to parturition for the determination of total antioxidant power. Briefly, after dilution, 10 μl of serum were added to “Ferric Reducing Ability of Plasma” (FRAP) reagent, incubated at 37°C for 30 min. and the absorbance was determined by Spectra Shell Microplate Reader at 595 nm. Our data shows a significant decrease (P<0.01) in total antioxidant capacity from T1 to T2 and T3. In conclusion, mares are subject to an increased oxidative stress at parturition and during the first week of lactation. Further researches are needed to evaluate the potential effects of this variation of the oxidative status on mares fertility.
Does the anti-Oxidative Status of Mares Change during the Peripartum Period? / Righi, Federico; S. E., Santini; Renzi, Marco; Bussolati, Simona; Basini, Giuseppina; Grasselli, Francesca; Quarantelli, Afro. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 41:(2006), pp. 362-362. (Intervento presentato al convegno REPRODUCTION IN DOMESTIC ANIMALS tenutosi a Portoroz - Slovenia nel 7 th - 9 th september 2006).
Does the anti-Oxidative Status of Mares Change during the Peripartum Period?
RIGHI, Federico;RENZI, Marco;BUSSOLATI, Simona;BASINI, Giuseppina;GRASSELLI, Francesca;QUARANTELLI, Afro
2006-01-01
Abstract
Free radical production is a biological phenomenon which occurs constantly during metabolic processes. Under normal conditions, Reactive Oxygen Species production is balanced by scavenger enzymes and antioxidant molecules. An increase of oxidative stress, which has been demonstrated in dairy cattle in particular stressfull conditions such as peripartum, can be related with decreased fertility. The aim of this study was to investigate the anti-oxidative status of mares around parturition. Blood samples were taken from 17 thoroughbred mares at days –24 (T1), 1 (T2) and +9 (T3) relative to parturition for the determination of total antioxidant power. Briefly, after dilution, 10 μl of serum were added to “Ferric Reducing Ability of Plasma” (FRAP) reagent, incubated at 37°C for 30 min. and the absorbance was determined by Spectra Shell Microplate Reader at 595 nm. Our data shows a significant decrease (P<0.01) in total antioxidant capacity from T1 to T2 and T3. In conclusion, mares are subject to an increased oxidative stress at parturition and during the first week of lactation. Further researches are needed to evaluate the potential effects of this variation of the oxidative status on mares fertility.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
