Background: Platelet-rich plasma is used in oral and maxillofacial surgery; however, its real efficacy is debated. Also, the in vitro effects on bone-specific functions are contradictory, understanding the mechanisms of action of platelet-derived factors could be the basis for their proper use in clinical applications. Methods: The functional parameters of osteoblasts (proliferation, alkaline phosphatase, collagen synthesis, and calcium deposition) were analyzed in vitro for 14 days in the presence of different concentrations (100%, 33%, and 11%) of platelet gel releasate (PGR). Results: Concentrations of 100% PGR and 33% PGR stimulated cells to proliferate more than 10% fetal calf serum. The effect on cell proliferation was dose dependent, and the addition of dexamethasone (dex) and ?-glycerophosphate (?-GP) reduced the proliferative effects. Alkaline phosphatase activity was stimulated by 33% PGR and 11% PGR after 7 days and was induced further by dex and ?-GP. Also, collagen synthesis, measured on day 11, was stimulated by 33% PGR and 11% PGR. Calcium deposition, evaluated after 7 and 14 days, was greatest in cells treated with PGR supplemented with dex and GP. The mineralization process increased with time; on day 14, calcium aggregates were observed in all cultures treated with PGR (100%, 33%, and 11%). Conclusions: PGR stimulated osteoblast proliferation in a dose dependent manner and, when used at 33% and 11%, induced maximum levels of alkaline phosphatase and collagen synthesis. Moreover, in the presence of dex and ?-GP, PGR stimulated the end maturative status of cells as expressed by the deposition of calcium nodules.

Dose-dependent effects of platelet gel releasate on activities of human osteoblasts / UGGERI J; BELLETTI S; GUIZZARDI S; POLI T; CANTARELLI S; SCANDROGLIO R; GATTI R.. - In: JOURNAL OF PERIODONTOLOGY. - ISSN 0022-3492. - 78(2007), pp. 1985-1991. [10.1902/jop.2007.070116]

Dose-dependent effects of platelet gel releasate on activities of human osteoblasts

UGGERI, Jacopo;BELLETTI, Silvana;GUIZZARDI, Stefano;POLI, Tito;SCANDROGLIO, Renato;GATTI, Rita
2007

Abstract

Background: Platelet-rich plasma is used in oral and maxillofacial surgery; however, its real efficacy is debated. Also, the in vitro effects on bone-specific functions are contradictory, understanding the mechanisms of action of platelet-derived factors could be the basis for their proper use in clinical applications. Methods: The functional parameters of osteoblasts (proliferation, alkaline phosphatase, collagen synthesis, and calcium deposition) were analyzed in vitro for 14 days in the presence of different concentrations (100%, 33%, and 11%) of platelet gel releasate (PGR). Results: Concentrations of 100% PGR and 33% PGR stimulated cells to proliferate more than 10% fetal calf serum. The effect on cell proliferation was dose dependent, and the addition of dexamethasone (dex) and ?-glycerophosphate (?-GP) reduced the proliferative effects. Alkaline phosphatase activity was stimulated by 33% PGR and 11% PGR after 7 days and was induced further by dex and ?-GP. Also, collagen synthesis, measured on day 11, was stimulated by 33% PGR and 11% PGR. Calcium deposition, evaluated after 7 and 14 days, was greatest in cells treated with PGR supplemented with dex and GP. The mineralization process increased with time; on day 14, calcium aggregates were observed in all cultures treated with PGR (100%, 33%, and 11%). Conclusions: PGR stimulated osteoblast proliferation in a dose dependent manner and, when used at 33% and 11%, induced maximum levels of alkaline phosphatase and collagen synthesis. Moreover, in the presence of dex and ?-GP, PGR stimulated the end maturative status of cells as expressed by the deposition of calcium nodules.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/1709639
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