Non-differentiated THP-1 cells can be infected by human cytomegalovirus (HCMV) Towne strain, which persists in these cells in a non-active (latent) form without undergoing a productive cycle. The same cells become permissive for HCMV lytic infection after induction of cell differentiation by treatment with 12-O-tetradecanoylphorbol-13-acetate. We used this cellular model to study the possible role of histone modifications in the control of HCMV latency. Using chromatin immunoprecipitation with antibodies against histone H3 acetylated or dimethylated in position K9, we demonstrated that in lytically infected cells the HCMV enhancer was associated with heavy acetylated but not dimethylated H3. In the case of latent infection, the HCMV enhancer was associated with neither acetylated nor dimethylated H3. HCMV genes encoding DNA polymerase (early), pp65 (early–late) and pp150 (late) proteins were associated preferentially with acetylated H3 in lytically infected cells and with dimethylated H3 in latently infected cells. These data strongly suggest that K9 methylation of H3 is involved in HCMV gene repression, while association of the above genes with acetylated histones is likely to be necessary for active transcription. It can be postulated that the same histone modifications are used to mark active and repressed genes in both cellular and viral chromatin.

Control of human cytomegalovirus gene expression by differential histone modifications during lytic and latent infection of a monocytic cell line / Ioudinkova, E; Arcangeletti, Maria Cristina; Rynditch, A; DE CONTO, Flora; Motta, Federica; Covan, Silvia; Pinardi, Federica; Razin, Sv; Chezzi, Carlo. - In: GENE. - ISSN 0378-1119. - 384:(2006), pp. 120-128. [10.1016/j.gene.2006.07.021]

Control of human cytomegalovirus gene expression by differential histone modifications during lytic and latent infection of a monocytic cell line

ARCANGELETTI, Maria Cristina;DE CONTO, Flora;MOTTA, Federica;COVAN, Silvia;PINARDI, Federica;CHEZZI, Carlo
2006-01-01

Abstract

Non-differentiated THP-1 cells can be infected by human cytomegalovirus (HCMV) Towne strain, which persists in these cells in a non-active (latent) form without undergoing a productive cycle. The same cells become permissive for HCMV lytic infection after induction of cell differentiation by treatment with 12-O-tetradecanoylphorbol-13-acetate. We used this cellular model to study the possible role of histone modifications in the control of HCMV latency. Using chromatin immunoprecipitation with antibodies against histone H3 acetylated or dimethylated in position K9, we demonstrated that in lytically infected cells the HCMV enhancer was associated with heavy acetylated but not dimethylated H3. In the case of latent infection, the HCMV enhancer was associated with neither acetylated nor dimethylated H3. HCMV genes encoding DNA polymerase (early), pp65 (early–late) and pp150 (late) proteins were associated preferentially with acetylated H3 in lytically infected cells and with dimethylated H3 in latently infected cells. These data strongly suggest that K9 methylation of H3 is involved in HCMV gene repression, while association of the above genes with acetylated histones is likely to be necessary for active transcription. It can be postulated that the same histone modifications are used to mark active and repressed genes in both cellular and viral chromatin.
2006
Control of human cytomegalovirus gene expression by differential histone modifications during lytic and latent infection of a monocytic cell line / Ioudinkova, E; Arcangeletti, Maria Cristina; Rynditch, A; DE CONTO, Flora; Motta, Federica; Covan, Silvia; Pinardi, Federica; Razin, Sv; Chezzi, Carlo. - In: GENE. - ISSN 0378-1119. - 384:(2006), pp. 120-128. [10.1016/j.gene.2006.07.021]
File in questo prodotto:
File Dimensione Formato  
Ioudinkova et al., 2006 - abstract.pdf

non disponibili

Tipologia: Abstract
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 68.92 kB
Formato Adobe PDF
68.92 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Ioudinkova et al., 2006.pdf

non disponibili

Tipologia: Altro materiale allegato
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 700.67 kB
Formato Adobe PDF
700.67 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1494146
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 79
  • ???jsp.display-item.citation.isi??? 79
social impact