A simple and rapid method for the determination of ochratoxin A (OA) in ham was developed using a basic methanolic extraction, immunoaffinity column clean-up and a fluorometric determination of the toxin contamination levels. A mean recovery of OA from ham samples spiked at levels from 0.7 to 9.7 mug kg(-1) was 83 +/- 6% using the fluorometric method, with a detection limit of 0.7 mug kg(-1). Recovery data were compared statistically with those obtained using reversed-phase high-performance liquid chromatography with acetonitrile-water-acetic acid (99:99:2) as mobile phase and fluorescence detection, commonly used for OA determination in food. A good correlation between the two analytical techniques was obtained. Both methods were successfully applied to 42 ham samples, 21 in the middle of the ripening period (after 6 months from the process beginning) and the other 21 at the end of the maturation, after 12 months. Twenty-seven samples (64%) showed an OA contamination level < 1.0 &mu;g kg(-1), the Italian Ministry of Health guideline. The maximum contamination level found was 2.3 &mu;g kg(-1). A good agreement (R-2 = 0.980) between HPLC and fluorometer analysis on naturally contaminated samples was obtained.

Ochratoxin A determination in ham by immunoaffinity clean-up and a quick fluorometric method / E. Chiavaro; A. Lepiani; F. Colla; P. Bettoni; E. Pari; E. Spotti. - In: FOOD ADDITIVES AND CONTAMINANTS. - ISSN 0265-203X. - 19:6(2002), pp. 576-582. [10.1080/0265203021012386 9]

Ochratoxin A determination in ham by immunoaffinity clean-up and a quick fluorometric method

CHIAVARO, Emma;
2002

Abstract

A simple and rapid method for the determination of ochratoxin A (OA) in ham was developed using a basic methanolic extraction, immunoaffinity column clean-up and a fluorometric determination of the toxin contamination levels. A mean recovery of OA from ham samples spiked at levels from 0.7 to 9.7 mug kg(-1) was 83 +/- 6% using the fluorometric method, with a detection limit of 0.7 mug kg(-1). Recovery data were compared statistically with those obtained using reversed-phase high-performance liquid chromatography with acetonitrile-water-acetic acid (99:99:2) as mobile phase and fluorescence detection, commonly used for OA determination in food. A good correlation between the two analytical techniques was obtained. Both methods were successfully applied to 42 ham samples, 21 in the middle of the ripening period (after 6 months from the process beginning) and the other 21 at the end of the maturation, after 12 months. Twenty-seven samples (64%) showed an OA contamination level < 1.0 μg kg(-1), the Italian Ministry of Health guideline. The maximum contamination level found was 2.3 μg kg(-1). A good agreement (R-2 = 0.980) between HPLC and fluorometer analysis on naturally contaminated samples was obtained.
Ochratoxin A determination in ham by immunoaffinity clean-up and a quick fluorometric method / E. Chiavaro; A. Lepiani; F. Colla; P. Bettoni; E. Pari; E. Spotti. - In: FOOD ADDITIVES AND CONTAMINANTS. - ISSN 0265-203X. - 19:6(2002), pp. 576-582. [10.1080/0265203021012386 9]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/1450319
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