CO rebinding kinetics after nanosecond photolysis of myoglobin encapsulated in wet silica gels exhibits an enhanced geminate phase that allows the determination of the microscopic rate constants and the activation barriers for distinct ligand docking sites inside the protein matrix. Using a maximum entropy method, we demonstrate that the geminate phase can be well-described by a biphasic lifetime distribution, reflecting rebinding from the distal and proximal sites. Microscopic rates and activation barriers were estimated using a four-state model.

Determination of microscopic rate constants for CO binding and migration in myoglobin encapsulated in silica gels / Sottini, Silvia; Abbruzzetti, Stefania; Viappiani, Cristiano; Ronda, Luca; Mozzarelli, Andrea. - In: JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL. - ISSN 1520-6106. - 109:(2005), pp. 19523-19528. [10.1021/jp054098l]

Determination of microscopic rate constants for CO binding and migration in myoglobin encapsulated in silica gels

SOTTINI, SILVIA;ABBRUZZETTI, Stefania;VIAPPIANI, Cristiano;RONDA, Luca;MOZZARELLI, Andrea
2005-01-01

Abstract

CO rebinding kinetics after nanosecond photolysis of myoglobin encapsulated in wet silica gels exhibits an enhanced geminate phase that allows the determination of the microscopic rate constants and the activation barriers for distinct ligand docking sites inside the protein matrix. Using a maximum entropy method, we demonstrate that the geminate phase can be well-described by a biphasic lifetime distribution, reflecting rebinding from the distal and proximal sites. Microscopic rates and activation barriers were estimated using a four-state model.
2005
Determination of microscopic rate constants for CO binding and migration in myoglobin encapsulated in silica gels / Sottini, Silvia; Abbruzzetti, Stefania; Viappiani, Cristiano; Ronda, Luca; Mozzarelli, Andrea. - In: JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL. - ISSN 1520-6106. - 109:(2005), pp. 19523-19528. [10.1021/jp054098l]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/1443710
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