Direct measurement of interleukin-33 (IL-33) in biological systems is critical for understanding its role in inflammatory diseases. In this work, we have developed a platform for the discovery and optimisation of fluorogenic biosensors that are built from scFv protein scaffolds. Our approach combined site-specific fluorophore labelling and deep-learning protein design to identify BS-7 as a biosensor for wash-free detection of human IL-33 in cell supernatants.
A platform to design and optimise fluorogenic scFvs for detection of interleukin 33 / Reese, Abigail E.; Karmakar, Utsa; Restori, Margherita; Hermoso, Marcela A.; Church, George M.; Kuru, Erkin; Vendrell, Marc. - In: CHEMICAL SCIENCE. - ISSN 2041-6520. - (2026). [10.1039/d5sc09634k]
A platform to design and optimise fluorogenic scFvs for detection of interleukin 33
Restori, Margherita;
2026-01-01
Abstract
Direct measurement of interleukin-33 (IL-33) in biological systems is critical for understanding its role in inflammatory diseases. In this work, we have developed a platform for the discovery and optimisation of fluorogenic biosensors that are built from scFv protein scaffolds. Our approach combined site-specific fluorophore labelling and deep-learning protein design to identify BS-7 as a biosensor for wash-free detection of human IL-33 in cell supernatants.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
