Most of the silicone oils used clinically in vitreoretinal surgery are composed of poly-dimethyl-siloxane. Sudden central visual loss in vitrectomized eyes soon after the removal of the silicone oil tamponade is an unexplained severe complication of this surgical procedure with incidence ranging from 1 to 30% [1]. Low-molecular weight components are the most likely responsible of a toxic effect on the retina leading to the visual loss [2]. In this study, for the first time, an in vivo assessment of contaminants in the 4 different types of viscous tamponade potentially responsible for retinal toxicity was performed. Firstly, an untargeted GC-MS approach was applied to identify the contaminants present in each tamponade used: both linear and cyclic siloxanes were identified in all the samples, being cyclic siloxanes already present in some of the commercial preparations. Then, a Box-Wilson Central Composite Design (CCD) followed by the multicriteria method of desirability functions [3] was applied to search for the optimal conditions for the headspace gas chromatography-mass spectrometry (HS-GC-(SIM)-MS) quantitation of the identified siloxanes. Extraction temperature (50-100 °C) and extraction time (20-60 min) were selected as main factors. A global desirability D=0,91 was calculated in correspondence to an extraction temperature and an extraction time of 100 °C and 20 min, respectively. The HS-GC-(SIM)-MS method was validated according to the bioanalytical method validation guidelines [4] and applied for the analysis of 42 patients categorized by the different type of tamponade used. Traces of hexamethylcyclotrisiloxane and octamethylcyclotetrasiloxane were observed in high percentages in all the groups examined. The correlation between the ocular concentration of siloxanes and the time of permanence in the eye may support a progressive passage of the substances into the retina
An in vivo gas chromatography-mass spectrometry evaluation of silicone oil contaminants and their possible correlation with retinal detachment / Ribezzi, Erika; Marraffa, Enrico; Bianchi, Federica; Riboni, Nicolo'; Mora, Paolo; Careri, Maria. - (2023). ( 11th MS J-Day Bari ).
An in vivo gas chromatography-mass spectrometry evaluation of silicone oil contaminants and their possible correlation with retinal detachment
Erika Ribezzi;Enrico Marraffa;Federica Bianchi;Nicolo' Riboni;Paolo Mora;Maria Careri
2023-01-01
Abstract
Most of the silicone oils used clinically in vitreoretinal surgery are composed of poly-dimethyl-siloxane. Sudden central visual loss in vitrectomized eyes soon after the removal of the silicone oil tamponade is an unexplained severe complication of this surgical procedure with incidence ranging from 1 to 30% [1]. Low-molecular weight components are the most likely responsible of a toxic effect on the retina leading to the visual loss [2]. In this study, for the first time, an in vivo assessment of contaminants in the 4 different types of viscous tamponade potentially responsible for retinal toxicity was performed. Firstly, an untargeted GC-MS approach was applied to identify the contaminants present in each tamponade used: both linear and cyclic siloxanes were identified in all the samples, being cyclic siloxanes already present in some of the commercial preparations. Then, a Box-Wilson Central Composite Design (CCD) followed by the multicriteria method of desirability functions [3] was applied to search for the optimal conditions for the headspace gas chromatography-mass spectrometry (HS-GC-(SIM)-MS) quantitation of the identified siloxanes. Extraction temperature (50-100 °C) and extraction time (20-60 min) were selected as main factors. A global desirability D=0,91 was calculated in correspondence to an extraction temperature and an extraction time of 100 °C and 20 min, respectively. The HS-GC-(SIM)-MS method was validated according to the bioanalytical method validation guidelines [4] and applied for the analysis of 42 patients categorized by the different type of tamponade used. Traces of hexamethylcyclotrisiloxane and octamethylcyclotetrasiloxane were observed in high percentages in all the groups examined. The correlation between the ocular concentration of siloxanes and the time of permanence in the eye may support a progressive passage of the substances into the retinaI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
