A novel therapeutic scheme is proposed to control bacteria that causes chronic lung infections replacing antibiotics with inhalable persistent light emission particles (LEP) such as ZrO2:Ti4+ particles developed by ICIQ group for this project. The system developed consist on exciting the LEPs with the conditions to achieve maximum emission spectra, then deliver the LEPs through an aerosol system in order to “deliver” the light emitted by the particles in order to excite bacterial endogenous porphyrins and therefore, inhibit bacterial growth by a photodynamic effect consisting of local production of 1O2. The work presented shows the relevant photophysical properties of LEPs characterized through several spectroscopic methods such as fluorescence spectroscopy, absorption spectroscopy and time-resolved fluorescence spectroscopy measurements to delve in the persistent and prompt luminescence properties of these particles. Preliminary experiments to obtain evidence of light effect in photodynamic killing of bacteria using S. aureus as a model of Gram+ bacteria and KI as an photodynamic enhancer were also done. Relevant photophysical properties of these materials such as excitation and emission spectra, intensity time profiles of persistent and prompt light emission were determined in this work. Also preliminary microbiological results shows that KI enhances the photodynamic killing effect of light inhibiting completely the growth of bacteria in this conditions.
Photophysical Characterization of ZrO2:Ti4+ Persistent Luminisecence Particles and Preliminary Studies of KI as Photodynamic Enhancer of Light Induced Bacterial Inactivation / Dibona-Villanueva, Luciano; Troglia, Sofia; Bresolí-Obach, Roger; Gulias Borau, Oscar; Gené, Santi; Martínez, Eugenia; Méndez, Maria; Palomares, Emilio; Nonell, Santi. - STAMPA. - (2022). ( 6th Photobiology School - ESP Casa della Gioventù Universitaria of the University of Padova, Bressanone, Italy 21-27/08/2022.).
Photophysical Characterization of ZrO2:Ti4+ Persistent Luminisecence Particles and Preliminary Studies of KI as Photodynamic Enhancer of Light Induced Bacterial Inactivation.
Sofia Troglia;
2022-01-01
Abstract
A novel therapeutic scheme is proposed to control bacteria that causes chronic lung infections replacing antibiotics with inhalable persistent light emission particles (LEP) such as ZrO2:Ti4+ particles developed by ICIQ group for this project. The system developed consist on exciting the LEPs with the conditions to achieve maximum emission spectra, then deliver the LEPs through an aerosol system in order to “deliver” the light emitted by the particles in order to excite bacterial endogenous porphyrins and therefore, inhibit bacterial growth by a photodynamic effect consisting of local production of 1O2. The work presented shows the relevant photophysical properties of LEPs characterized through several spectroscopic methods such as fluorescence spectroscopy, absorption spectroscopy and time-resolved fluorescence spectroscopy measurements to delve in the persistent and prompt luminescence properties of these particles. Preliminary experiments to obtain evidence of light effect in photodynamic killing of bacteria using S. aureus as a model of Gram+ bacteria and KI as an photodynamic enhancer were also done. Relevant photophysical properties of these materials such as excitation and emission spectra, intensity time profiles of persistent and prompt light emission were determined in this work. Also preliminary microbiological results shows that KI enhances the photodynamic killing effect of light inhibiting completely the growth of bacteria in this conditions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
