Two mutant strains of pseudorabies virus (PRV) were selected from the virulent 86/27V virus treated with chemical drugs. The viruses, named 6A1 and 6C2, respectively, appeared to be unable to express thymidine kinase function, as demonstrated by the autoradiography test. They showed a reduced virulence for some susceptible animal species (chickens, mice, rabbits, calves, lambs and piglets) and virus was isolated sporadically. The mutant viruses appeared to be able to protect animals against infection with the virulent strain of PRV. At gross, as well as at histological examination, no lesions in apparatus, system and tissues were detected in pigs inoculated with 6A1 and 6C2 viruses. By contrast, rabbits treated with 6C2 mutant strain presented lymphomononucleated cuffs, microgliosis, and neuronophagia in some areas of the brain. This focal spreading, together with the absence of neuronal necrosis and intranuclear inclusions, suggest an infection induced by a modified strain of PRV. © 1995 Blackwell Verlag GmbH
A study of two mutant strains of pseudorabies virus (PRV) unable to express thymidine kinase (TK) function / Ferrari, M.; Gualandi, G. L.; Boldini, M.; Corradi, A.; Losio, M. N.; Bergonzini, P.. - In: JOURNAL OF VETERINARY MEDICINE. SERIES B. - ISSN 0931-1793. - 42:1-10(1995), pp. 1-11. [10.1111/j.1439-0450.1995.tb00675.x]
A study of two mutant strains of pseudorabies virus (PRV) unable to express thymidine kinase (TK) function
Corradi A.Membro del Collaboration Group
;
1995-01-01
Abstract
Two mutant strains of pseudorabies virus (PRV) were selected from the virulent 86/27V virus treated with chemical drugs. The viruses, named 6A1 and 6C2, respectively, appeared to be unable to express thymidine kinase function, as demonstrated by the autoradiography test. They showed a reduced virulence for some susceptible animal species (chickens, mice, rabbits, calves, lambs and piglets) and virus was isolated sporadically. The mutant viruses appeared to be able to protect animals against infection with the virulent strain of PRV. At gross, as well as at histological examination, no lesions in apparatus, system and tissues were detected in pigs inoculated with 6A1 and 6C2 viruses. By contrast, rabbits treated with 6C2 mutant strain presented lymphomononucleated cuffs, microgliosis, and neuronophagia in some areas of the brain. This focal spreading, together with the absence of neuronal necrosis and intranuclear inclusions, suggest an infection induced by a modified strain of PRV. © 1995 Blackwell Verlag GmbHI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
